DreINT0046715 @ danRer10
Intron Retention
Gene
ENSDARG00000057025 | chrna2b
Description
cholinergic receptor, nicotinic, alpha 2b (neuronal) [Source:ZFIN;Acc:ZDB-GENE-041001-99]
Coordinates
chr20:19919379-19919689:+
Coord C1 exon
chr20:19919379-19919423
Coord A exon
chr20:19919424-19919579
Coord C2 exon
chr20:19919580-19919689
Length
156 bp
Sequences
Splice sites
5' ss Seq
CAGGTATGT
5' ss Score
9.8
3' ss Seq
TTTTGTGGCCTCTCGTTCAGGAA
3' ss Score
8.52
Exon sequences
Seq C1 exon
GACGAAAAGAATCAGATGATGACAACAAACGTCTGGCTCAAGCAG
Seq A exon
GTATGTGTGGCAATAAAGGTAATATGTGGAATTTAGGGGGGAGAAAGGTTTAAATAATCCAGCATTCAGTAAATAAATTGGCAAAAGCTCAAATAATGTAAGACATTTTACAGTGAATTAATGCTAATTTGTTATATTTTGTGGCCTCTCGTTCAG
Seq C2 exon
GAATGGAATGATTATAAACTGCGCTGGAAGCCTTCAGACTACGACAATGTGACTTCCATCAGGGTTCCATCAGAACTCATCTGGGTTCCGGATATAGTTTTGTATAACAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000057025:ENSDART00000136317:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0293118=Neur_chan_LBD=FE(6.8=100)
A:
NA
C2:
PF0293118=Neur_chan_LBD=FE(17.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACGAAAAGAATCAGATGATGACA
R:
TGTTATACAAAACTATATCCGGAACC
Band lengths:
154-310
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]