DreINT0055706 @ danRer10
Intron Retention
Gene
ENSDARG00000035507 | ddx31
Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 [Source:ZFIN;Acc:ZDB-GENE-030131-3973]
Coordinates
chr5:29126275-29126603:+
Coord C1 exon
chr5:29126275-29126325
Coord A exon
chr5:29126326-29126463
Coord C2 exon
chr5:29126464-29126603
Length
138 bp
Sequences
Splice sites
5' ss Seq
CAGGTTTGA
5' ss Score
5.11
3' ss Seq
GTGTCATGTTTCTGTTTTAGTAC
3' ss Score
10.02
Exon sequences
Seq C1 exon
GATGTAGCTGCACGTGGTTTGGATTTGCCTCAGGTTACTTGGATTGTTCAG
Seq A exon
GTTTGATTTCTTCATGTTCAAGATTTTATCATTTGCATAATTGCCAGTGTGCAACATATAAACAAATTTATTCAATTTCTTCACAAAACTTCTAAATATTCTGTATATTTTTAAATGAGTGTCATGTTTCTGTTTTAG
Seq C2 exon
TACAATCCTCCAGTTTCTGCAGCAGAATATGTTCATCGTGTGGGCCGCACAGCTCGAATCGGAGCTCAGGGTAGCAGCCTGCTCTTTCTCACCCCCTCCGAGACCGCTTTTGTGGATGTGCTGGCCAATCACAACATCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035507:ENSDART00000148314:15
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0027126=Helicase_C=PU(42.1=94.1)
A:
NA
C2:
PF0027126=Helicase_C=PD(52.6=42.6),PF047579=Pex2_Pex12=PU(2.6=8.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTAGCTGCACGTGGTTTGGAT
R:
TGATGTTGTGATTGGCCAGCA
Band lengths:
187-325
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]