DreINT0055714 @ danRer10
Intron Retention
Gene
ENSDARG00000035507 | ddx31
Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 31 [Source:ZFIN;Acc:ZDB-GENE-030131-3973]
Coordinates
chr5:29115803-29116161:+
Coord C1 exon
chr5:29115803-29115873
Coord A exon
chr5:29115874-29116096
Coord C2 exon
chr5:29116097-29116161
Length
223 bp
Sequences
Splice sites
5' ss Seq
CAGGTGGGG
5' ss Score
6.92
3' ss Seq
TTTATTTATGTGTTGTACAGGGA
3' ss Score
6.78
Exon sequences
Seq C1 exon
TGTCCAGAAACAGACCATACCAGTGCTGATGTCTGGTAAAGATGCTGTAGTGCGCTCCCAGACTGGATCAG
Seq A exon
GTGGGGTTTTTGATGTAAATAAATTTTATTAAATTAAAATATAAAAATATAAGATGTATAACCGAATTACTAGAGTAACAAAATGTTGAGTGTCATATTAAACATTAAATTGAATTTTATATTGAATGTGTGGTTTGAAAACTATAAAAATAGAAGTCATGAAAGTGTTTGTTAGAGAAAGAATGTGCTGTATGTAAGGTTTGTTTATTTATGTGTTGTACAG
Seq C2 exon
GGAAAACATTGGCATATGGGATTCCAATGGTTCAGTTTTTACAAGCGATTCAACCGAAAGTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035507:ENSDART00000148314:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0027024=DEAD=FE(13.5=100)
A:
NA
C2:
PF0027024=DEAD=FE(11.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCAGAAACAGACCATACCAGTGC
R:
CTTGACTTTCGGTTGAATCGCT
Band lengths:
133-356
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]