DreINT0056048 @ danRer10
Intron Retention
Gene
ENSDARG00000058666 | dennd2da
Description
DENN/MADD domain containing 2Da [Source:ZFIN;Acc:ZDB-GENE-061013-542]
Coordinates
chr8:12965961-12966379:-
Coord C1 exon
chr8:12966310-12966379
Coord A exon
chr8:12966039-12966309
Coord C2 exon
chr8:12965961-12966038
Length
271 bp
Sequences
Splice sites
5' ss Seq
CTGGTAAAT
5' ss Score
6.06
3' ss Seq
ACAAATCTTTTCTTGTTTAGCCT
3' ss Score
6.39
Exon sequences
Seq C1 exon
TGAAACATTCTCTTTCGTGCTTACAGATGTTGATGGAACAAGGAAGAATGGATACTGTAGGAGGCTGCTG
Seq A exon
GTAAATGCTGACTGGACCCATTAGTGACACTTCCTGTAGTTTTTGCTCAGAACTTAACATTAATTAGTTTGAACAGAATCTCTGAATCTAAGTGTTGACACATTTGCACAATGTCTTTTAAACAAACGCCTACATTACCCTGTCAACATACTGTAGCAACAGGAAAACACCTCCAACATTTTCTTCAGAAAAGTCTAGATATTTTTGTAATAAATGAAGAAGGTTATTACTGTGTGATTTGATTGTTTCTAACAAATCTTTTCTTGTTTAG
Seq C2 exon
CCTGATGGGAAGGGTGCTCGTCTGCCTGAAGCCTACTGCATCATCAGTAATGTGGCCTGTTTTGGGCTCTTTTCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058666:ENSDART00000140969:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0345613=uDENN=FE(32.4=100)
A:
NA
C2:
PF0214116=DENN=PU(10.4=73.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAAACATTCTCTTTCGTGCTTACA
R:
CTTGGAAAAGAGCCCAAAACAGG
Band lengths:
148-419
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]