DreINT0056545 @ danRer10
Intron Retention
Gene
ENSDARG00000014439 | dgkza
Description
diacylglycerol kinase, zeta a [Source:ZFIN;Acc:ZDB-GENE-111104-3]
Coordinates
chr7:38825848-38826260:+
Coord C1 exon
chr7:38825848-38825959
Coord A exon
chr7:38825960-38826060
Coord C2 exon
chr7:38826061-38826260
Length
101 bp
Sequences
Splice sites
5' ss Seq
CTGGTATGT
5' ss Score
8.14
3' ss Seq
GTTGTTTTTTGTGACTTTAGGCC
3' ss Score
6.37
Exon sequences
Seq C1 exon
GTACTGTGCGGGCACAATGCCATGGGGCAATCCGAGTGAGCATCATGACTTTGAGCCTCAGCGTCATGATGATGGCTGCATTGAGGTTATTGGATTCACAATGACATCACTG
Seq A exon
GTATGTTTCACCTCACTGGATGCATTTGAAGTGAGTTTTCAAATGAAAGGGTCTATTCTTAAGTCAACAGATGTGCCCTGCGTTGTTTTTTGTGACTTTAG
Seq C2 exon
GCCACACTGCAGGTCGGAGGTCACGGTGAGAGGTTAAATCAGTGTCGCGAAGTCACCCTCACCACCTTTAAGTCAATCCCCATGCAGGTGGACGGGGAGCCTTGTAAACTAGCACCATCTGTTATTCACATTTCTTTGCGAAACCAGGCCAACATGGTGCAGAAAACCAAGAGACGTACATCTATACCACTGCTAAACGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014439:ENSDART00000093009:20
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.211 A=NA C2=0.149
Domain overlap (PFAM):
C1:
PF0060914=DAGK_acc=FE(23.4=100)
A:
NA
C2:
PF0060914=DAGK_acc=PD(20.9=49.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAATCCGAGTGAGCATCAT
R:
TTTTCTGCACCATGTTGGCCT
Band lengths:
253-354
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]