DreINT0059667 @ danRer10
Intron Retention
Gene
ENSDARG00000062083 | dph7
Description
diphthamide biosynthesis 7 [Source:ZFIN;Acc:ZDB-GENE-070410-57]
Coordinates
chr5:24577396-24579053:-
Coord C1 exon
chr5:24578966-24579053
Coord A exon
chr5:24577491-24578965
Coord C2 exon
chr5:24577396-24577490
Length
1475 bp
Sequences
Splice sites
5' ss Seq
CAGGTGACT
5' ss Score
6.6
3' ss Seq
TGTGTGTGTGTCTCCAGTAGCAG
3' ss Score
4.61
Exon sequences
Seq C1 exon
GTGCCATGTACCAATATCTGAGCGCCCATTATTGGGAATGGCCACTGCGAGTGGAGAAATCCAGCTTTACAAGCTTATCGAAGCTCAG
Seq A exon
GTGACTAATTACACTCATGCACAATCAGGACTGTTCTAAATATACTGTAATTAACAGGTCATCTCTACAACATAGACTATTATGTCTTGCTATAGTAATAAAGAAAATTATTAAAGAAGTTCATTTTTTAAATAAATATCTAAGCATCACATTTAAATGTTAAGCATCAGTAGACAGTTCATATTTTGTATTTTTTTTTAATACACAATCTGCATAAACGTGATATTTTTATCTGTGGAAAAACAATAGTTATTGTTAGGGAAAATTTTAAATGAAATTTTTACTTGCAATTGTTACAGTTAATTTGAAATGTATAAATAATATTATTTTAATTATGGAAGATATTTCTTAAGCTTATTAAAATCAATATTAATACAAGAGGGGTAGAGGGAGAGAGAGAGACTTTTAACAATCTCTTTATTTACAACCATTCCTTTAACACATTAATGCAAAGTCAAAAGTAAAAAGGGAGTGAAATAATGCACACAAATATACATCCTAGAGCCAGTTAACCCACAATCATTTACCAATCAAACAAGGATTCCCAAACAACTTTCATGACCAATTTCACAGACACATTAATTAATACACTATTTAGATTTAAAAGTGTGCAAATTATCAATCAATGTATAATACACATATTCTATTTTAATACAGGGTTTATAAGATCTCTAAACACCAAAATAGCGTCAGTCAACCCCAGACCAATCAGTTTTCTTTTTTATGACAACAAAATAGCCGTTTCTGCTTGTCCAAACATGGACTTGCATCTTGTTCCTGGGTAAAAAAAAACTGGGTGTAAAAACTTTCCCTTGTGAAAGAGAGAGAAAGGGCTTTAAAACAACAAAAGCATGTTGGCCTCAGCAAGAAAGTCGCTGGTTCCAGCACCGTCTGGGTCAGTTGGCATTTCTGTTTGGAGTTTGCATGTTCTCCCTGTGTTTGCCTGGGTATTCTTCAAAGACATGCGCTATAGGTGCATTGGGTAAGCTAAAATTGTCCATAGTGCATGAGTGTGTATGGGTGTTTCCCAGTGATGGGTTGCAGCTGAAAGGGCATCTGCTGCGTTAAACATATGCTGGTGGTTCATTCCGCTGTGGCGACCCCTGATGGATAAAGGAACTGAGGCGAAAAGAAAATTAATGAATGAACCTTTAAAACAAGAACTTTCAATGCTATTTATATATATCATATTTATTTCACATATAATATCATATTTTATTCAGCTTTTATGACTTGACACAACCCTTGCATACTGATTTAATTTTAATTAATTAATTTTAAGTTTAATTACATCTGTTTGTCTCATTTGTAGATTGTGTTGCATAAAAATGAACGCTAAATTATTATTTTTGTATACAGTATATAGAATTTAAGGTAATCCTTTAGTCCCCTTAGAAGCTGCTTAGTGTTTCTACTGTGTGTAAGATGTTTTATTGTGGTGTTTGTTCTTCAATATGTGTGTGTGTCTCCAGTAG
Seq C2 exon
CAGGGCTCCAGTGGTCTGGAGTGTGAGCTGAGCACAGATTTGGGTCCTGACAGACTGGCCTTGTCTTTAGACTGGTCCACAGGGCGAGGCGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062083:ENSDART00000089339:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.024
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCATGTACCAATATCTGAGCG
R:
CTCTCGCCTCGCCCTGTG
Band lengths:
182-1657
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]