DreINT0063334 @ danRer10
Intron Retention
Gene
ENSDARG00000100666 | ep300a
Description
E1A binding protein p300 a [Source:ZFIN;Acc:ZDB-GENE-080403-16]
Coordinates
chr12:18817657-18818294:-
Coord C1 exon
chr12:18818055-18818294
Coord A exon
chr12:18817746-18818054
Coord C2 exon
chr12:18817657-18817745
Length
309 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAG
5' ss Score
8.04
3' ss Seq
TTTTGTTTGTTTCTTTACAGCTT
3' ss Score
11.3
Exon sequences
Seq C1 exon
GACTATTTTGACATTGTGAAAAATCCAATGGACTTGTCTACTATCAAACGAAAGCTTGACACAGGCCAGTACCAGGAGCCATGGCAGTATGTAGATGACATCTGGCTTATGTTCAACAACGCCTGGCTGTACAATCGTAAGACATCACGGGTCTACAAGTACTGCTCCAAACTGGCGGAGGTCTTTGAGCAAGAAATTGACCCAGTCATGCAGAGCCTTGGCTACTGTTGTGGGAGAAAG
Seq A exon
GTACAGTTGAGAACACAGGACTGTTTGTACAAATTAAATTAGTTTTATAAGATACTGCCTATTTTACAAATCGATATTTTCTTTTTTTTGTGGACAGAATAAATGAGAGTTTGGGGCTGATTCTGTAATGATCATTAGAGAGTAGGTGTTGAGCTGGCAGAGGGTGATCATAATGGGGTTTTTAGTATTAGTGCAGATTCAAGTACATGTTGATCTGAAAGGGACCTAAAATTGATCTGATATTTTATATATAGGTATGTTTTTGCCAAAATAACATTGGCTAAGTTTTTTTTGTTTGTTTCTTTACAG
Seq C2 exon
CTTGAATTTTCTCCCCAAACTCTGTGCTGCTATGGGAAACAGTTATGCACTATACCACGAGATGCTGCTTACTTTAGTTATCAGAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100666:ENSDART00000172574:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.013 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0043920=Bromodomain=PD(64.4=70.0),PF060018=DUF902=PU(28.6=15.0)
A:
NA
C2:
PF060018=DUF902=PD(66.7=93.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACATTGTGAAAAATCCAATGGACT
R:
TGTTCTGATAACTAAAGTAAGCAGCA
Band lengths:
320-629
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]