DreINT0069639 @ danRer10
Intron Retention
Gene
ENSDARG00000044809 | fpgs
Description
folylpolyglutamate synthase [Source:ZFIN;Acc:ZDB-GENE-040426-2781]
Coordinates
chr21:6020332-6020590:+
Coord C1 exon
chr21:6020332-6020434
Coord A exon
chr21:6020435-6020512
Coord C2 exon
chr21:6020513-6020590
Length
78 bp
Sequences
Splice sites
5' ss Seq
AAGGTGATT
5' ss Score
5.88
3' ss Seq
AGAGCATATGTTTATTTCAGCCT
3' ss Score
3.04
Exon sequences
Seq C1 exon
GCGTCCTTGGGTGTGCGGTATCTCGTCTTTAGGTATCGATCACACTAGCATTCTGGGAGACACCATTGAGAAAATCGCCTGGCAGAAAGGAGGGATTTTTAAG
Seq A exon
GTGATTAGTGTGTTGTGACTGTAAACTCAAAGCTTGGAGATTTTGAAGGTATTCAGTGAGAGCATATGTTTATTTCAG
Seq C2 exon
CCTGGAGTTCCTGCGTTCACAGTCAAACAGCCTGACGGGCCGATGAAAGTGCTTCAGGAGAGAGCAGAAGAAGTTGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044809:ENSDART00000141607:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.038
Domain overlap (PFAM):
C1:
PF082457=Mur_ligase_M=FE(16.0=100)
A:
NA
C2:
PF082457=Mur_ligase_M=FE(11.8=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCTTGGGTGTGCGGTATCTC
R:
TCTGCTCTCTCCTGAAGCACT
Band lengths:
168-246
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]