DreINT0078064 @ danRer10
Intron Retention
Gene
ENSDARG00000039452 | hk1
Description
hexokinase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-2848]
Coordinates
chr13:22857286-22857591:-
Coord C1 exon
chr13:22857496-22857591
Coord A exon
chr13:22857386-22857495
Coord C2 exon
chr13:22857286-22857385
Length
110 bp
Sequences
Splice sites
5' ss Seq
GGAGTGAGT
5' ss Score
6.05
3' ss Seq
GTGAAATGATGTACTTACAGGAT
3' ss Score
5.39
Exon sequences
Seq C1 exon
GCTGTTTTGCTGACATGGACGAAACGCTTCAAGGTTAATGGAGTAGAGGGAATGGATGTAGTGAAGCTTCTCAATAAGGCCATTAAAAAACGTGGA
Seq A exon
GTGAGTATTATCAAGTCATTCTTTCTATTGACCATCTGGTTGATGTACAGGTGGATAATATTAATGTATTATTTAAAGAGGGATTTAATAGTGAAATGATGTACTTACAG
Seq C2 exon
GATTACGAAGCAGATATAATGGCTGTTGTCAATGACACTGTTGGAACTATGATGACCTGCGGATTTGATGACCAGCGATGTGAAGTCGGCATTATCATAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000039452:ENSDART00000057638:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=FE(15.0=100)
A:
NA
C2:
PF0034916=Hexokinase_1=PD(11.2=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTGTTTTGCTGACATGGACG
R:
ATGATAATGCCGACTTCACATCG
Band lengths:
194-304
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]