DreINT0078099 @ danRer10
Intron Retention
Gene
ENSDARG00000038703 | hkdc1
Description
hexokinase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9801]
Coordinates
chr13:22894651-22895055:-
Coord C1 exon
chr13:22894956-22895055
Coord A exon
chr13:22894835-22894955
Coord C2 exon
chr13:22894651-22894834
Length
121 bp
Sequences
Splice sites
5' ss Seq
TAGGTAAGA
5' ss Score
9.14
3' ss Seq
GGTTCTGTCTGTTTCCTCAGGTA
3' ss Score
9.95
Exon sequences
Seq C1 exon
GATCTTGATGTGGATGTCCTGGCGATGGTCAATGACACAGTGGGGGCCATGATGACCTGTGGATACGATGACCAGAACTGTGAAGTGGGGGTTATTGTAG
Seq A exon
GTAAGATTTTTAGACTGAACCATTTTCCTAATATATCTTTAATTAAATCTAATCATAAAAATGCATTACTTCAAATAGGAGAAGACATCTCAAAAGTTAAAGGTTCTGTCTGTTTCCTCAG
Seq C2 exon
GTACTGGCACCAATGCGTGTTACATGGAGGAAATGCGGCACATTGACCTGGTGGAGGGAGATGAAGGAAGAATGTGTATCAATACTGAATTGGGGGCATTTGGAGATGATGGTGTTCTTGATGATTTCATTACAAGCTTTGACCGTGAAATTGATGCCGCCTCTATTAATCCAGGAAAACAGCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000038703:ENSDART00000056523:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0034916=Hexokinase_1=PD(11.2=67.6),PF0372711=Hexokinase_2=PU(3.3=23.5)
A:
NA
C2:
PF0372711=Hexokinase_2=FE(25.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCGATGGTCAATGACACAGT
R:
AGAGGCGGCATCAATTTCACG
Band lengths:
244-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]