DreINT0084824 @ danRer10
Intron Retention
Gene
ENSDARG00000061990 | kcnh4b
Description
potassium voltage-gated channel, subfamily H (eag-related), member 4b [Source:ZFIN;Acc:ZDB-GENE-121214-54]
Coordinates
chr12:13426151-13426636:+
Coord C1 exon
chr12:13426151-13426227
Coord A exon
chr12:13426228-13426374
Coord C2 exon
chr12:13426375-13426636
Length
147 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAA
5' ss Score
6.82
3' ss Seq
ATACAATTTCTCCATAACAGATG
3' ss Score
6.94
Exon sequences
Seq C1 exon
GCTCTGGTCAGGTGAATGTTTCCCTGCTACAGGAAACAAAGGAAGTGAGAGAAACTATTGATCAGCTTAACAAGAAG
Seq A exon
GTACAATCACATTATAAAAGTTCATGAAAATAGTACACATGATGACCTCAATCACTGTGTAATTACAATATCTTTTTAATTTAGTGAAAAATAATTCACATTATATTAAATGATGTAATAATTTTGAATACAATTTCTCCATAACAG
Seq C2 exon
ATGGGCAACCTGAAAGAGGAAGTGGCTAATCTAACAAAAGATCTTCATCATATAATGCACCTGCTTCAAGCACATATAGCTGCTCGTCATTATGCTGCACCTTTCTCCTCCTGTCCCTATGGAGTCCACATGATGTCCAGTGCTGCTGTCAGCATGTCTCCAACCTACATTTTGGGTTCCAATATCCACATGCAGCACAAAGACCATGTTGTCTCTGAGGGTCTTTTGAGATCCACATCATGGAACCATAGCAGAGTTAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061990:ENSDART00000089042:14
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=1.000 A=NA C2=0.273
Domain overlap (PFAM):
C1:
PF153971=DUF4618=FE(32.5=100)
A:
NA
C2:
PF153971=DUF4618=PD(37.7=33.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCTGGTCAGGTGAATGTTTC
R:
GGTTGGAGACATGCTGACAGC
Band lengths:
242-389
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]