DreINT0094975 @ danRer10
Intron Retention
Gene
ENSDARG00000017229 | megf10
Description
multiple EGF-like-domains 10 [Source:ZFIN;Acc:ZDB-GENE-080506-1]
Coordinates
chr10:16168792-16170645:+
Coord C1 exon
chr10:16168792-16168912
Coord A exon
chr10:16168913-16170481
Coord C2 exon
chr10:16170482-16170645
Length
1569 bp
Sequences
Splice sites
5' ss Seq
CAGGTAATG
5' ss Score
9.43
3' ss Seq
CGTCATTGCAACTCTGTCAGGGT
3' ss Score
3.32
Exon sequences
Seq C1 exon
GGCTCAGACTGTGCCATAGCCTGCCCTCCGGGTACATATGGCATCAACTGTACTTCTCTGTGTTCTTGTAAAAATGGAGCCATTTGTTCACCCATCGATGGATCATGTTCTTGTCAAGCAG
Seq A exon
GTAATGCTCTTCAGTTCTCATCTTTAGGATCCACGTTCCTGCAGAGTTTAGCTCCAGCCCTGATCCCCACATGTAACTTTCTAATTCAGTTCAAGTTTTACTGGATTGGAGCAAAATCACTGCAAAAATGGTTTCAGTGGACAGGGGATCTCCTACAGTTGAAGTCAGAAATATTAACTTTAATGATATAATAGCCTCCCTATATATTGTCCTCCAATTTTGGTTTAAAGGAAATATTTCATTACTAAACATAATAGTTTTAATAATACATTTTTTTATGACTGATTTTTTTTTATCTTTGCTATAATGACATTACATAATATTTTACTAGATATTTTTCAAGATACTAGTATTCAGCTTAAAGTGTAAGGTTTCATGGTAACACTTTATAATATCTACACACTATGTATCATTTACCAAGCATTGGCATCTAGTGAATTCATTATTTGTTAAGCATTAACTCTACATTTATAAACGTTAGTAAGCAGTTTATAACTGCAGCTACAAATGCTGCATTCTTGACTTATAAGCACCTATATAATGTGTTTATTAATTGTATTTTCATACTTAGTAAATGATTTATTTTTTATTACTAAATTAAATATTGCATTATTTACAAACCATTTGTATTTAAGAGTAGTTGAGGGTTTTTAGGATCATTCAGAATGAGTTAGTAAATGATTACTAAACTATTAAAATCAACATTTATTTATCTTATTATGCAGGCATATACAAATAGTTAACAAATATGTTAATAAATGCTTTATTAACTCAACTTCATGCAGTTTTGTGACTTTATCTAAAGTGAGGACTATTTATGCTTTATAAATCCATTATAAATGACAATTAAAGGCTCTGAATCAAATGAACAATAATCTGTGCTATCTTTTCTAAAATGTAAAATTACTGTAAAGTTTAAACATTGCCAAATAACAGGAGTGTCAAAATACAACATAAAACTGGATAAAACAACAACAATAATATAATAATTTAACAATATAATAAGATGCATTGTTAAAACTCTACAGTGATTTTATTTTAGATGAGGTTGCAAAGATTTATTTTCCATAACCAGTTTCATTTGTGTATAGGAACGAATAAAGTCATTCTTCCTGTTTAGAATTTAACTGAGCCTTTATTTGTCATTTATAAAGGATTTATAAAGCATGAATAGTCCTCACTTTAGATTAGGTCACAAAACTAGATGAAGTTGAGGTAATAAAACATTTATTAACATATATAGTAACCATTAATATATGCTTGAATAATAAGAAATATAAATGTTGATTTTAATAGTTTATTAATCATTTACTAACTAATTCTGAATGATCCTAAAAACACTCAACTACTCTTATATACAAATGGTTTGTAAATAATGCGATACTTAATTTAGTAATGAAAAATAAATCATTAACTAAGTATGAAAGTATAAATATTAAGCACATTTTAAATATGTTTGTAAGTCAAGAATAGAGTTTTACTGTTAATTATTGTTAGAAATATTTATTAATAGTCTATGTCATTTCTTAACTCTATACTTTCTCCTTGTCGTCATTGCAACTCTGTCAG
Seq C2 exon
GGTGGCATGGGGTGGACTGCTCAATTAACTGCCCCAGTGGAACGTGGGGTTTGGGCTGTAACCTGTCATGTGTGTGTGGTAATGGAGGCGCATGCAATGCTCTTGATGGCAAATGCACATGTACTCCAGGCTGGAGAGGAGACAGATGTGATCAACATTGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000017229:ENSDART00000043936:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF126612=hEGF=PD(23.1=7.3)
A:
NA
C2:
PF079748=EGF_2=WD(100=58.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTCAGACTGTGCCATAGCCTG
R:
ACAATGTTGATCACATCTGTCTCCT
Band lengths:
280-1849
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]