DreINT0095032 @ danRer10
Intron Retention
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21210120-21210451:-
Coord C1 exon
chr23:21210323-21210451
Coord A exon
chr23:21210252-21210322
Coord C2 exon
chr23:21210120-21210251
Length
71 bp
Sequences
Splice sites
5' ss Seq
GAAGTGAGT
5' ss Score
7.97
3' ss Seq
GATTGTGTTGTGCCCTGCAGTGT
3' ss Score
7.92
Exon sequences
Seq C1 exon
TTTGTTTACCTGGTTCCTATGGGAAGCGTTGTGCTCATCGATGCCAGTGTCCTCATGGAACCTCCTGTAACCATGTGACTGGACAGTGTGGTTGCCCAGCGGGATTAACTGGTAGTGGCTGTGAGCGAA
Seq A exon
GTGAGTGATCGTCTCTTAATCTCATTGCACTCATGTGTTCAGTGAAACAGTGATTGTGTTGTGCCCTGCAG
Seq C2 exon
TGTGCCAGTCAGGTACGTTTGGGGTAAACTGTAATCAGGTGTGCCAGTGCTCTGAGCTCAACCAGCTCTGCCACCCCGTCACTGGTGTCTGTTACTGTGCTCCCGGATACTATGGCAGCAAGTGTGAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199:ENSDART00000143206:25
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(34.8=36.4)
A:
NA
C2:
PF0005319=Laminin_EGF=PU(67.3=77.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCATGTGACTGGACAGTGTGG
R:
TTGCTGCCATAGTATCCGGGA
Band lengths:
180-251
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]