DreINT0095044 @ danRer10
Intron Retention
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21237570-21238709:-
Coord C1 exon
chr23:21238572-21238709
Coord A exon
chr23:21237693-21238571
Coord C2 exon
chr23:21237570-21237692
Length
879 bp
Sequences
Splice sites
5' ss Seq
TGGGTGAGT
5' ss Score
8.73
3' ss Seq
TTATTGCCTTTGCTTTGTAGATC
3' ss Score
9.2
Exon sequences
Seq C1 exon
GTATTGAAATGGAGATTGTCAATACCTGTGACTCTGATAATGGTGGCTGTTCACATCGCTGTCAGCATGGTGTTCTTGGGCCTGTCTGCAGCTGTAATCATGGTTATCAGCTCGCCGAAGACCTCAAAACCTGTTTGG
Seq A exon
GTGAGTATGCAGGTGTACAAAAGCGTACATACATTTTAAAGAGTTTTTTTAATTAAACAATTTCTTGGATTTGTGCATCTGCACTGATAAACCTTCAAAAGCACCAGTCCTCATGTACATAAAGCAAAATCAAATGCCTGCCAGTCATAAGTTTGGCATTATTTTTTGGGAGGATCAACTTTAATGTTTTATTTTGGACAAAAATCCTTTTTCTCTTACTTACCAAGGCTGCATTTATTTAAATTAATAACAAATGCAATACACAGTATCATTGTGCTGTGTTATTTCGGTTATAAAATAATATCTTTTGTTTTGATAAAGTACATTATGTTTTAATATATTGTATTATATGATTTCTAAACAAAGCTGAATTTTTAGCATCGTTACTACAGTATTCTTTGTGTCACATTATTCTTCAGAAATCATTTTAAATTGTTGATTTGCTGATATATGGCTATATATGAACATATATATGTAATTATAGATTATTTATTTGAACAAATATAGGTACATATTTTTGCAATATTTTAAAATATATGTTGCATATATGACATATAGTTAATGTGAAATACAAACAATAACTTACAGTATATGTCATTCATGTCTTTTGCATTTTTGCCATATATCAGATTTCTATATGGACAATTATTAATTCTCAGTCAATCAATATTTGTAATAATCAAACATGTTTATTAAGTCTTAAATCATCATATTAGACTTATTTTTGAAGGATCCTGTGACACTGAAAACTGGAATAATGATGCTAAAAATCTCCTTTAAATCACAGGAATAAATGAAATACTAAAATGGCATGTTATATGATTCCCTGTGCTAGTTTTTATTTTTAATCCTTTTTCATTTATTGCCTTTGCTTTGTAG
Seq C2 exon
ATCTGGAGGAATGTGAGGAAGGAACGTCATGCTGTGAGCAGGATTGCACAAACTACCCTGGAGGGTATGAATGCTACTGCAGGGCAGGTTACAGACTCAATGCAGATGGATGCGGATGTGACG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199:ENSDART00000143206:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF146701=FXa_inhibition=WD(100=76.6),PF0764510=EGF_CA=PU(0.1=0.0)
A:
NA
C2:
PF0764510=EGF_CA=WD(100=95.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AATGGAGATTGTCAATACCTGTGACT
R:
CGTCACATCCGCATCCATCTG
Band lengths:
254-1133
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]