DreINT0095045 @ danRer10
Intron Retention
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21237359-21237692:-
Coord C1 exon
chr23:21237570-21237692
Coord A exon
chr23:21237482-21237569
Coord C2 exon
chr23:21237359-21237481
Length
88 bp
Sequences
Splice sites
5' ss Seq
ACGGTACTT
5' ss Score
6.66
3' ss Seq
TTTGTGTTGTGTTTGTGTAGATG
3' ss Score
8.41
Exon sequences
Seq C1 exon
ATCTGGAGGAATGTGAGGAAGGAACGTCATGCTGTGAGCAGGATTGCACAAACTACCCTGGAGGGTATGAATGCTACTGCAGGGCAGGTTACAGACTCAATGCAGATGGATGCGGATGTGACG
Seq A exon
GTACTTCACAACCTGTTTACTGATGACCAACATTAAAGGAGAGCGAGGAGACATACAGTATTGTTCACTTTGTGTTGTGTTTGTGTAG
Seq C2 exon
ATGTGGACGAGTGTGCGTCTGATAGAGGAGGCTGTGAACACACTTGTCAGAATACGGCTGGCTCATATCACTGCTTCTGTCGGTTTGGATATCGACTTGGTGAGGACAGACACTCCTGCATTT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199:ENSDART00000143206:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0764510=EGF_CA=WD(100=95.2)
A:
NA
C2:
PF146701=FXa_inhibition=WD(100=85.7)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAGGAACGTCATGCTGTGAGC
R:
GCCAGCCGTATTCTGACAAGT
Band lengths:
167-255
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]