DreINT0098268 @ danRer10
Intron Retention
Gene
ENSDARG00000103308 | mst1
Description
macrophage stimulating 1 [Source:ZFIN;Acc:ZDB-GENE-020806-3]
Coordinates
chr6:53420743-53421159:-
Coord C1 exon
chr6:53421044-53421159
Coord A exon
chr6:53420858-53421043
Coord C2 exon
chr6:53420743-53420857
Length
186 bp
Sequences
Splice sites
5' ss Seq
AGGGTGAGG
5' ss Score
7.13
3' ss Seq
CATGTTTTGTATATGTTCAGTAT
3' ss Score
5.42
Exon sequences
Seq C1 exon
AGCTTTCAACTATGAGTTTCGTCCATCATTGGTGTGTAAACATCTGCCCTGGGTGGGCGACGGAGACAATGCAGACGTGAAGAGAAACGTGAACTGTGACCTCTATGAGATGAAGG
Seq A exon
GTGAGGAATTGCTAGCTAAAATGTTGCTATAGTAAATTGGGTCTAAAGTATTACAAAGTTACTTTTAAACATTATGAGGACATTGATAACATTGTGGGATGTTATAGAAAATACAGCTCTCTAACATTACATTAAATAACCGCTAGTTATGATTGTAATGTTCACACATGTTTTGTATATGTTCAG
Seq C2 exon
TATATGTAAGGAAATGCATTGTGGGTAAAGGAGAAGACTATCGAGGGAAAGTGTCGACAACTACAAGTGGACGAACATGTCAACAGTGGTGGTCAAAATTCCCTCATGACCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000103308:ENSDART00000162791:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0002421=PAN_1=FE(47.0=100)
A:
NA
C2:
PF0002421=PAN_1=PD(0.1=0.0),PF0005113=Kringle=PU(42.9=84.6)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTTTCAACTATGAGTTTCGTCCA
R:
TGGTCATGAGGGAATTTTGACCA
Band lengths:
228-414
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]