DreINT0099829 @ danRer10
Intron Retention
Gene
ENSDARG00000100972 | myh11b
Description
myosin, heavy chain 11b, smooth muscle [Source:ZFIN;Acc:ZDB-GENE-101124-2]
Coordinates
chr3:36560184-36560426:-
Coord C1 exon
chr3:36560334-36560426
Coord A exon
chr3:36560248-36560333
Coord C2 exon
chr3:36560184-36560247
Length
86 bp
Sequences
Splice sites
5' ss Seq
TTTGTGAGT
5' ss Score
5.91
3' ss Seq
GAATGCTCTTAACTTCTCAGGGT
3' ss Score
7.16
Exon sequences
Seq C1 exon
GGAGAGCTTGAGAAGCAGCTCCTGCAGGCCAATCCCATTCTGGAGGCATTTGGAAATGCAAAGACCATCAAAAATGATAATTCTTCAAGATTT
Seq A exon
GTGAGTTTACACTTCATTGCTTCAATACTGAAAATTTGTTGATTACCTTTATAATCAACTAATAAAGAATGCTCTTAACTTCTCAG
Seq C2 exon
GGTAAATTTATCAAAATCAACTTTGACAACACCGGCTACATCGTTGGAGCAAACATTGAAACGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000100972:ENSDART00000166896:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.5=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(3.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGCAGGCCAATCCCATTCTG
R:
ACGTTTCAATGTTTGCTCCAACG
Band lengths:
136-222
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]