DreINT0100042 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40754223-40754550:-
Coord C1 exon
chr6:40754463-40754550
Coord A exon
chr6:40754341-40754462
Coord C2 exon
chr6:40754223-40754340
Length
122 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAAA
5' ss Score
2.44
3' ss Seq
ATTAACTCTCTTTATTTTAGGTC
3' ss Score
11.21
Exon sequences
Seq C1 exon
GAAAATTTGGGCAAATTGATGACCAACCTGAGAAGCACTCATCCACATTTTGTGCGCTGCTTGATTCCAAATGAGTCAAAGACTCCAG
Seq A exon
GTCAAATTTTTGACACAACTTTTTTTAAAATACAGAAAAGCATGTGACATATACTCTTTGTTAAAATAACAATGATCATCCTCTCTAAAACAATGATTTATTATTAACTCTCTTTATTTTAG
Seq C2 exon
GTCTTATGGAGAACTTTCTGGTCATCCACCAGTTGCGTTGTAATGGTGTGCTGGAGGGAATCAGAATTTGCAGAAAGGGTTTCCCCAGCAGAATCCTCTATGGTGACTTTAAGCAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:18
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(4.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCAAATTGATGACCAACCTGA
R:
TCTGCTTAAAGTCACCATAGAGGA
Band lengths:
196-318
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]