DreINT0100054 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40750874-40751266:-
Coord C1 exon
chr6:40751148-40751266
Coord A exon
chr6:40751071-40751147
Coord C2 exon
chr6:40750874-40751070
Length
77 bp
Sequences
Splice sites
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
3' ss Seq
TGGAAATTTGTATGTTTAAGGCC
3' ss Score
4.79
Exon sequences
Seq C1 exon
GTGAAATGGGTCGTCAACTAGAGGAAAAAGAGTCTTTAGTTTCTCAGCTGACTCGAAGCAAACAGGCTTACACTCAACAGATTGAGGAGCTGAAGAGGCAAATTGAGGAAGAAGTCAAG
Seq A exon
GTGAGAAATTAACAATTTTTATAGAACAAGTTCATGAACTAATCTATTGAATAACTCTGGAAATTTGTATGTTTAAG
Seq C2 exon
GCCAAAAATTCCCTGGCACATGCTGTCCAATCATCACGACATGACTGTGATCTGCTCAGAGAGCAGTACGAGGAGGAACAGGAGGCCAAAGCTGAACTTCAGCGAAGCATGTCAAAGGCCAACAGTGAAGTAGCTCAATGGAGAACCAAATATGAGACCGATGCTATTCAGCGTACTGAAGAACTCGAGGAAGCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:29
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.775 A=NA C2=0.773
Domain overlap (PFAM):
C1:
PF0003816=Filament=PD(6.2=45.0),PF0157614=Myosin_tail_1=FE(4.5=100),PF131661=AAA_13=FE(18.0=100),PF0003816=Filament=FE(12.6=100)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(7.6=100),PF131661=AAA_13=FE(30.0=100),PF0003816=Filament=FE(21.0=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTGACTCGAAGCAAACAGG
R:
GTTCAGCTTTGGCCTCCTGTT
Band lengths:
171-248
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]