DreINT0100069 @ danRer10
Intron Retention
Gene
ENSDARG00000001993 | myhb
Description
myosin, heavy chain b [Source:ZFIN;Acc:ZDB-GENE-081124-1]
Coordinates
chr6:40757174-40757398:-
Coord C1 exon
chr6:40757371-40757398
Coord A exon
chr6:40757286-40757370
Coord C2 exon
chr6:40757174-40757285
Length
85 bp
Sequences
Splice sites
5' ss Seq
TACGTAAGT
5' ss Score
9.27
3' ss Seq
AATGTATGTTTCATTCTCAGTGG
3' ss Score
7.55
Exon sequences
Seq C1 exon
ATCGTGAAAATCAGTCTATCCTGATTAC
Seq A exon
GTAAGTATTTTCCTTCAGATGCTAAAGTGTTACTGTGTGGTAAAAATAAAGAAATTGTGAATCCTAATGTATGTTTCATTCTCAG
Seq C2 exon
TGGAGAATCTGGTGCAGGAAAGACTGTAAACACCAAACGTGTCATCCAGTACTTTGCGACAATCGCTGTGGCTGGCAAGCAGAAGCAAGAGCCTATTCCTGGAAAAATGCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000001993:ENSDART00000156295:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.053
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(1.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(5.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCGTGAAAATCAGTCTATCCTGA
R:
TGCATTTTTCCAGGAATAGGCTCT
Band lengths:
138-223
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]