DreINT0101020 @ danRer10
Intron Retention
Gene
ENSDARG00000099871 | myo7aa
Description
myosin VIIAa [Source:ZFIN;Acc:ZDB-GENE-020709-1]
Coordinates
chr18:3125306-3127191:+
Coord C1 exon
chr18:3125306-3125448
Coord A exon
chr18:3125449-3126980
Coord C2 exon
chr18:3126981-3127191
Length
1532 bp
Sequences
Splice sites
5' ss Seq
CAGGTAGAA
5' ss Score
6.68
3' ss Seq
TTTTTCTGTCTCTCGTCCAGCTT
3' ss Score
9.43
Exon sequences
Seq C1 exon
GGCATTTATGGCCGTTTGTTTGTGTGGATTGTTGAGAAAATCAACGCAGCCATTTATAAACCTCCATCTCTGGAGCTGAAAGCAGTGCGAAGATCCATCGGCCTATTAGATATCTTCGGCTTCGAGAACTTCATGGTTAACAG
Seq A exon
GTAGAAAACATTGCATGCAGATACTTTAACATGAACAAATATTCACTTATGCCTATGTTGTGCATATCACAATTGGTGATAATAGGGCCTGTTTACTTACTTTTTACAATTTGCTGGTCTAACTGTGGATGATTTAAAGCCAAAATGCTTTGTTCTTCTCTTCTTTTTAATCCAAATTCAATTCCTGCTGAGTTGACTTGACTTTTTGTATTAATTTATTTGTTATTTATGCATTAATAATTACTGCAATTTTTAATTGAATTGAATACAGCTTAATACATTAAGAAATATTAAATAATTTATTAATTGTTTTTATATTAATTTAATATTAAGTGGAACAATAATTTTATAATACATATACATTTTACTTGAATTATTTAAAAAGTTCCTCATTTAAAATAATAAGTAAGAAATTGTATATTTAATACATTTTTATTTTCAATAAATGAAATTTAATCAACCAAATGCATATACATTTTATTCAATTTACATGCAAATATGTTTAATAATTACTATGCTGGATTATATATTAATATTGAAATAACTTTCGTTCTTTAATACATTTGCTAGTATAAAATTGTATTATAGTAATATAGTTTATTATGTAATATATTTAATAAAATACATTGAAAATAAGAACTAATAGCTTTTAATATGTAAAAAGTAAATTTATTACATAAATTAATTACATAAAATCTCAAATACAGTGATCAGCATATATAAGTACACCCCTCACAAATCTATCTTTTAAATTCATATTTTTAATAGGAAGCTATACAATATTATATTTGCGCATATACATTAGATTATTTAGTACCGAAACCAAATCTGGAGCTTATCTAACACCATAACTAACGATAACAATCCAAAAACTATTACAGCCAAATTTATATGTTATAGAATAATGTTAAATGCAAATTTAAAAAAGAGGAAAAATCAAGAGATGCAAAAAAAATAAAAAATGCAAATTAAAAATTGTAGCTGAAATTTTGTAGGTTGTATTTTTTTCAATATTTTGCATAAATTTCAATGTATTATCTTTCTAATTCTAAAGATGTTCTGTGACTAAAATAATATTTTGCATGAATTTAAATGTATTATTCTTCTATTTCTAAAGATATTCTGTGACTAAAATATTATTTTAATAAATATATCTGTATAATAAATCTGTCGTTTTTAAATGCACCAAAATACATTGCCTATATTCACTGAGAAATAGATAGAAATATTTATTTTCAAAATGGGGTTCAGTGTTGAGCACTGCATATATTTCATATACTGCATATCTATTTCAACTTTGGTCGAAAATGAATAAAGTCAGTAACATCAATCAGTAAATAATAATTTATATTAATCACTGTAATTTTTTTGCATAATAATAATATTAATATTAATTAATATATAAATATAAATAATAATAATAAATAAATAATTTGTTAAAACCTGACACCCATCTTATGTGTTTAGATTTAGATTCTGTCTGTTTGATGTATTTGTGATTTATTTATTTATTTTTTCTGTCTCTCGTCCAG
Seq C2 exon
CTTTGAGCAGCTGTGCATTAACTTTGCCAATGAAAACCTGCAGCAGTTTTTCGTCCGTCATGTGTTCAAGCTGGAGCAGGAGGAATATAACCTGGAGAACATCAACTGGCAGCACATCGAGTTCACAGACAACCAGGACGCTTTAGACATGATCGCCATAAAGCCCATGAACATCATCTCGCTCATCGACGAGGAGAGCAAGTTTCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000099871:ENSDART00000166980:11
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(7.1=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(10.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGCCGTTTGTTTGTGTGGAT
R:
GGAAACTTGCTCTCCTCGTCG
Band lengths:
342-1874
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]