DreINT0101065 @ danRer10
Intron Retention
Gene
ENSDARG00000044632 | myo7ab
Description
myosin VIIAb [Source:ZFIN;Acc:ZDB-GENE-091110-4]
Coordinates
chr21:22238812-22240736:+
Coord C1 exon
chr21:22238812-22238954
Coord A exon
chr21:22238955-22240525
Coord C2 exon
chr21:22240526-22240736
Length
1571 bp
Sequences
Splice sites
5' ss Seq
CAGGTTAGT
5' ss Score
8.02
3' ss Seq
TTCATTCCTGCTGTGTGTAGCTT
3' ss Score
7.09
Exon sequences
Seq C1 exon
GGAATCTATGGGAGGTTGTTTGTGTGGATAGTGGATAAAATCAATGCTACCATCTTCAGGGCTCCTTCCACAGAAAACAGGACCGTTCGCAGGTCTATAGGCCTGCTCGACATCTTCGGTTTCGAGAATTTCACTGTTAACAG
Seq A exon
GTTAGTTTTGGTAAATAGAAAAATTTGGGTTGTTTTTATTTCCCCAAGTGCAACAGCTCTGCTCAGAGTGTAGTCACACTTTGCTTAATACAAAATCTGGCGTGATTGCTCAGTTAATGCGGTTCATTTTATTAAGGCGCGAACACTGCCACACACAAACACAACAAACAAGTAGTGACTAAGACTGCAGAGAAAATGAATCTCTGTCCAAATAAATTTTGAAATATGAATGCAACATGAACCAACATGAACTGCACCCGCATGTTTTGAGCTTGCGTCTGAGAGCTGCTGTGAGTCAGGATGTAAATTTATTCTGCAAGATGATTTAAAATTACTTCTTAATTATGGAACTTCACATTCCTTAACATTAGATTTTTAATGAGGCCTGGACCTCAGTGACCTTAATGCTGGCTATGGGCTTGCTCAAGTATCTGCCTTTTTTTCTTGTCTCCTTGTCTCCTTCTCCTTCTTTTGTTTGTGAATAACACAAATAAATAAATGAATAATTTCATTTAAATAAAATAAATATTTATTTATTTCAGTGGTTGAACTAGGTATTGAATTCCTGGATCAAAACAATTGCAAGCGCAGGTTGCCAGATTGAGGACCAACAAGGCTGATTTAAACCGTGTTCTAAATAAAAGCTACAGCACACAATAGAAGGAATATTTTACATATTAAAGGAGTTTTCGCCAACACCTCAAATTGATATATAGGAAACGGCTTCTTATAGCTGAACAACAGAAAACTTTGACAATGATCACATTAGGTACACCTCATGTGCCTTATTTAGTGTTAAATGCTAATAATGTGAGTTTGAATCCCCTTTTACATGACATTTATTGCCATACTACTAAAAACAACAGCAGATAGTTCACCTCAGATCTTGAAAATAAAATAAATAAAAATTAAACCATTTTGAAATTGGTTTGTGCTGTGACTCAGTTCAAACCAACACGTGATTGAGCATGTACATTTAATAATGTTAAAGAGGTTTAATATGCATTAATTAGGGTATAAACCTTACAGTTTCGTGAGTGCATATTCTGTGCTTCTGGACGGCTGTATTTACATTTTGTCTTAATTCGTTTGGTGCAAATAGCCAAATTGCTTGTCACTGCAAATCAAATGCATTCTTTGAGAGCCTTCCTGACTAATTGAATGAAATATGCTGTTTTCCATAAAGGCAACCCGGAGTGCTTAAATATAATTGTCTAAACTGGCATTGGACGGGTAAAAGGACCAAAACAACGACAGCGTTCTGACGCGGAAAACACATTTTCAAAGCTAAATAAAGTTCAGCATTGTTATTTAGATAAACAAGAGTGTTCACTTGGCATGTTTCTTAAATATCTGCAAACATAGTATGGCATTTTTATGCTTTATAGAAGAGTCAAAAACTTACATACAGCACCGTTAACCTTAGCATTGCTTTTGAAATGCTCTGCTATTTTCACCTCAAACTTGGAAAATCTAACATTTCTAATGTCTATGATGGTTAAAATTCTGTTTTAAAGATATTGTCTGAGAACCAAACCAGTACTTCTCTTGTTCATTCCTGCTGTGTGTAG
Seq C2 exon
CTTTGAGCAACTGTGCATCAACTTTGCTAACGAGAACCTGCAGCAGTTCTTCGTCCACCATGTTTTCAAGCTGGAGCAGGAGGAGTATAACCTGGAGGACATCAACTGGCAGGACATTGAGTTCACCGACAACCAGGACGCCCTAGACATGATTGCCATCAAACCTATGAATATCATTTCCCTCATTGATGAGGAGAGCAAGTTCCCAAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044632:ENSDART00000148019:10
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006316=Myosin_head=FE(7.3=100)
A:
NA
C2:
PF0006316=Myosin_head=FE(10.7=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGGAGGTTGTTTGTGTGGAT
R:
TGGGAACTTGCTCTCCTCATCA
Band lengths:
343-1914
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]