DreINT0103384 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23026858-23027591:-
Coord C1 exon
chr9:23027490-23027591
Coord A exon
chr9:23026963-23027489
Coord C2 exon
chr9:23026858-23026962
Length
527 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAAG
5' ss Score
9.65
3' ss Seq
TGTGTTTCCTCAATGGACAGGGA
3' ss Score
6.17
Exon sequences
Seq C1 exon
AGGTCCTACCGTGCCAGCTATGAGAAGTCTAAGGATAAGTACACCATAGTTGTAGATGACCCTAGAAACGTCTTGGCTAAGGAGAACAGCAAGATGAGTCAG
Seq A exon
GTAAAGCTACCAGCTGCAGCTGCCTCTACGGGTCCGTCGCCTGCCACAGGGGCCTGAGCATGAGGTCATCTTCAGTCCACTGGATGACTATGTCGCATGGCTACAACGTCACTTGATTGTGTCACTTTCTCTCCACTGATTTAGGTTTCCCTTTAAGTGACATTCAAAAAAATAATTTTTTGAGTGTGCACCGCTACTTCCAAATCTCTGTGCTCCAGATAATGCTTTGTCTGAAAGTGCGGTAGTTTGAGTGATATTCACAGTTGCTCAGTTAAATTTTGGTAATGAAAGTCAAACATTCTCCCACAGATTTTTTTATTATTTTCACCATATTGACAAACAGAAACATGATTGGTTTTCAAGCGACTACTGTATGAGCTTAAAATAGGGTTACTCTTGGGAAATCAGATATCTGGAGTACACAGTTTGGAACAGAACAACTCTTCTTGGACTTCAGTATCCACTTTCTGTCTCTGTCTTTTATTTGTGGCATGATTTGTGCATCTGTGTGTTTCCTCAATGGACAG
Seq C2 exon
GGAAAATATAAATGCAAGGCCAAGGAGTTACTCAAGGGTGGCTGCAATGAGCTGCTCCGTCCCGACATTCTCGTTGCCATGTACAACTCTGGCATGTGGAGTAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:106
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=32.4)
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTCCTACCGTGCCAGCTAT
R:
CTTACTCCACATGCCAGAGTTGT
Band lengths:
206-733
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]