DreINT0103403 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23013261-23015602:-
Coord C1 exon
chr9:23015492-23015602
Coord A exon
chr9:23013372-23015491
Coord C2 exon
chr9:23013261-23013371
Length
2120 bp
Sequences
Splice sites
5' ss Seq
GAGGTAACA
5' ss Score
8.1
3' ss Seq
TGTTCTTCTCCGCCCTGTAGAAA
3' ss Score
9.74
Exon sequences
Seq C1 exon
AAAGAGTATAAGAAGGACTTTGAGATGACAATGAAAGGAAAGAACCTCTCTGGGTTGGAGGTCACCCCTGCCATGATGCATGTCAGACACGCCACCAAAATTGTTAGCGAG
Seq A exon
GTAACATGAAGCTCTTTATATTCTTTCTTTCATCCTGGTAATAATACTAAGACCACATACACATGATAATTCTTACATGAGTGAAGTTTCCGGTTACACCTGAGTAGGTTATGGTTAGTATGCATGAACCATCCTCTCGTTGTAATACTCTCTCAGTAATTCCCTTCCCCTTCCAACATCTCTGTGTGTGTGTGCGCGTGTTTTATGTTGGTGCTGGTTTTAACAAGGTTCCCACAGTCACTGGCACTGCCTGGAAATATTAGGGAATTTAATTTTAGTCCGCCATTTCCAGGCTTCCAGAAGCTCGAATTTTGAGCCAAATAAAATGAAAAGTTAAAAAAATATGATTTTTTTAATATAGTGAATAAGTTAAGTCGCTTGTTAAGTCGTGATGTTTGAAATACTGTTTCCGGGTCCAAACCGCTACTAATTTGAATTGAGAAAATATTTGTTTTTGACCACTTTTTAGTCACAACACACAATAAAGTGAATTTTGTATAAAATCATGATGTACATAACTCTACATAAATCTAGACTTGCTGCCTCAGAGACACATATTTTATCGGCCATCGGATATTAAGCATGGTTATATATATTGCGATTGTGATTATCGAAAGTATTACATCGCTTTGTAAACCATTATTATTACTAGTTTGTTGAATGCCCCCATTTAATTTGATATAGTGCTTACAGGCCTGTAGCCAGGGGGGGTTTAGGTGGTTCGAAAGATCCACCCCTCACTGGCAAAAGTCCAGAATTTGTCCCATAGGCCAACATGAGCTCATTTGTCCTATTTTGTCTGCCATGCCATCATAGTGAAAATATCCCGTCAAAAAAAGTTTTAAGACCAAGTGAAATCCTCTGTTGGATGTCGTTTTGGTGTGACTTCAAGCTACAAAGCTACAACATCTAGCTGTGCAACAAAACATACAAAAAGAAAATATGTAACAAGTAACTTTTATTCCAAACCTTGGACCTTATTCCTGAAACAAAAAATCACCCATAAAAAGGTGTGAAGCGCCAAAACGGCCCATATTAAAATGAATTTGAATACCATTTTGGCTATTGTTGTTATCCACTTTGTGCTTTTTTGGTTATGATGACCATCTGACAATTCATTTAAGCTAAAATAGTGCATACTATGTCAGTAAAATGCTGTATTTTGATCTCATAATTAAATATGATTCTAAATGAGGCATATTACCGCAACTGCTACACTTTTTAAAAAAAAAGAACCACCCCTTTCACCAGGCTGGCTACGGGCTTGGCTTGGACCTGGAAGCTGCTTTGGTCACACTTGACAACCATATACATAAAACATTGGGCCCTCTCTTCAAAATAATTAGTAATGATTTCAAAAATTGTAATGAATGACCAGATATTCGATCATAATTCACTGATCAAAAGGTGTGGGAACCTTGTTGTTAAGGTCTTCTTTGGTGAATTCTCATGTTTTTTTTTCTGTTTTTCCATTGTACTTAAATGTACTGAGCATGTATCTAAAATAACACTTTATTATTTTATTATTTTGTTGTTTTCCTCTTTGCTCTTGAGTACATTTAATTTTTTCTTTACCCATTAGCATGATCATTATTTTTATTTGATTTGATTTTCTTTATTTAATATTAAGGCATTACCATTCTTTTCTTTCTTTACCATTTATTTACTATTACCATTACCATTTTCATTTAGCTTTTTTTTTTTACATTTTCAATTTTACATTTATTTATTTTATTTGATTTATTATTATGTCATTTTATCTAACCTCTTAAGATCTTGGTTTGGCTTTAATGTCACATGATTGCTGCTACATAAAGAATGTACTTGTAAAAGTGTCTCAGACAATATTTTTGTCAAAATTCTTGCCTGTGTTTTTTGATAATTATGTATACGTCTTTATCAGGTTGCTTTTCAAACTCAGCAGTACATTTGCTCTAAGTAAATCATATTTAGTTACTTACTACTATTAACAACAATGCAATGATTCTGTTTCGTAAACCCAGAGCAAATGCATGTTGATGTTGAACTGATACTGATTGCAGACAGTACTCCTCCAGTACTGCGTCTGTGTCTGTGTGCGCTTTGTGTCTGCAGTTTTTCTGTTCTTCTCCGCCCTGTAG
Seq C2 exon
AAAGAGTACAGAAGGGATCTAGAGGAAGGGGTGAAGGGTAAAGGGCTTACAGTGCTGGAGGAAACTCCTGAATTACTGAGAGCAAAGAATGCTACTCAGATCCTATGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:123
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.411 A=NA C2=0.196
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
A:
NA
C2:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]