RnoINT0099955 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37682361-37683046:-
Coord C1 exon
chr3:37682936-37683046
Coord A exon
chr3:37682472-37682935
Coord C2 exon
chr3:37682361-37682471
Length
464 bp
Sequences
Splice sites
5' ss Seq
GAGGTAGTA
5' ss Score
4.45
3' ss Seq
ATTTCATTTTTGTAATATAGAAA
3' ss Score
5.68
Exon sequences
Seq C1 exon
AAAGAATATAGGAAAGATTACGAAGAGTCCATTAAAGGCAGAAACCTGACGGGCCTGGAGGTCACGCCAGCATTGTTACATGTCAGACATGCAACCAAAATAGCGAGCGAG
Seq A exon
GTAGTAACCACGTGGTCTTCCCCTCCCCCTTCACCCAATAATCACACTAATGACTCATTGCTGTGCTCCTGCCTGGGTTCACCTGACTCAGGCTTCCAGTTGGCATCCCCATGTTTGGTACTAGTGTCTAATCCATGGCCCGTTTTAATTCTTCTAATTTTTAAAATGGAATATCTAATAAAACATACCTACCTTAGTATCCTGTAGGGAAAAAAATCTAATAAAACATGCCTACCTTAGCATTCTATTAATTCCCAGTCTTGTATTTAATTATTAACCTCAACTGTGAACCATACACCCTGCATTTACTCACGTTTTTACTTACGCAACCACGGACTGCTAAAAATTGGAATAACTGTCTCATTTATTCATTCACTTGTTTTGGTTTGGTTCTGGTTCTTTTCACCTAAGAGAATGATCCGGGCTTTCTTGTCAGTTTTGTTGATTTCATTTTTGTAATATAG
Seq C2 exon
AAAGAGTATAGGAAAGACCTAGAAGAAAGCATCCGGGGCAGAGGTCTCACTGAAATGGAAGATACTCCCGAAATGCTGAGAGCCAAGAATGCAACTCAAATCCTCAATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:95
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.378 A=NA C2=0.838
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=29.7),PF0088013=Nebulin=PU(57.1=43.2)
A:
NA
C2:
PF0088013=Nebulin=PD(35.7=27.0),PF0088013=Nebulin=PU(55.2=43.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TAGGAAAGATTACGAAGAGTCCATT
R:
CTCATTGAGGATTTGAGTTGCAT
Band lengths:
214-678
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]