DreINT0103404 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23011116-23013371:-
Coord C1 exon
chr9:23013261-23013371
Coord A exon
chr9:23011230-23013260
Coord C2 exon
chr9:23011116-23011229
Length
2031 bp
Sequences
Splice sites
5' ss Seq
GAGGTAGGA
5' ss Score
8.24
3' ss Seq
TGTCTGACCACTTTCTGCAGAGA
3' ss Score
6.71
Exon sequences
Seq C1 exon
AAAGAGTACAGAAGGGATCTAGAGGAAGGGGTGAAGGGTAAAGGGCTTACAGTGCTGGAGGAAACTCCTGAATTACTGAGAGCAAAGAATGCTACTCAGATCCTATGTGAG
Seq A exon
GTAGGACGGCTTAAGCTCATCTGATTTTTCTAACGCCACTTCCTCAACACACTAATCAAGCAGAAAGTAGTCAGTTTTCCTCCTGCTAATGAGAAAACTAATCAAGCTAATGGACCTCCCTATGATGCGCCATCTAACCAGGTTTGGTAAAGCAAATCAGCTCAACCCACTGAGACTCCCACTAAATGTCATTTTTCCTTTAAGTTGTAAGTGGTCTCCCTTATTCTTTTCTTCACAGCTAATATCTTCTAATCTACTGCTTTTATTGAGTTTGTAGCCATCAGGAGCTCTGGGTAACACTTTACTTCAAGGAGTGTTTATAAGACTGTCATGACACCTTTATAGTCATGACATGATTATATTTACATGATTGTACAAGAACGCCTATGTTAAGTTGCTATGACACAATCTAAATTGTAAAAGTGCTATAAAAATAAACAAGAATTGAACTGAATGACACATATGAAGATGACATGGACCCTTTTGACAAGACTGTAATGACGAGTTTAACGGTCATCATAATCTTAAATCCTTGACAGTTTTTAATATTTAGTTTTTTTAAATGTACCAACATTTATATGCATTTATGAATGTATTTTATCATCTTTATGTCAAATTACAAAAAAACAAATTGCTGCTTATGCGAAATGTTTCTAATGCAGTGTCTATGGGGCTGAGAGTAAATTTTTATGTTATTATTGTCATCAGTCTCATACTATTTCTTTTATTTTTCTAAAAGTCTTAATAACACCATTGTAGAGTTTTTCTTTTATTATTTTAGCAAATAGGATTGTAAAAAAATATTTTTGTACTCTCCTATTCACTGCGCTCTAAGTTTACCCAACTATGATGCCTTCCGCTACTGAGAAACCCGGAAATGTGAAAAGGGTCTATTTTTTGAGATGACAACTTAACAAAGAAATACATCATAAGATGTCAGTGTCTTTGTCATGACAACTTGACATTACTAAATTAGCTTGTCATAAATCTGTCATAAACATTATTGTCATAATTTTGTCATGAATATTTTCTGACCACTTTTTGTATCATTCAATTCTTTTTTTACGACATTGGAAATGGTCTGTTATCTGGCCGTATGTAGGAGGAAATAAAAAAAAAAACATGAAAAATTAATTTTGGGCCACTGTAGTTGAGGTCAAGAAAAATATTAATGACACTGTTATTAAATTATATGACAGATTACTAGCACATGAGAAATTTTTATGACATTTTTAGTTTGTTCCATTGAAAAAAAAGTAGTCAGAAATTATTTATGACACAAATATGTCAATCATGTTTATGACGTATTTATGACAAGTTATGTTGTCTTGGTAATGTCAAGTTGGCATGACAAAGAAACTGACATGTTTTGTCTTGGTAATATGTATACATGTCTAGATAATGTGTAAAAGAAAACAACCAAATTAATTCCTGCATGTGCATCAAAAAGTCATGTGAATTTGATTTAACAGATTTGATAACAAAAATGGGCAGGATAGGATGCCATTAATGGACAAACCAGCAGATCTACCATATTGTAAAATAACTGAAACATTTTTAAAAATCCTTTAGGCAAAATCCTTAGCCACTCCACTCCAAATAGAGCGGCCCTCCAAAAGACACTGCATTTTCATTGTGTTTTGAGGTGCAAGTAAATTAGTATGAAAAAGGGCCCACATTGGACATTCTTCTATTGCTGCAAATTCCATTTTTCTTTTTGATATTTGGTGCCAATTTAATAAGAAATGAAAACTCCAATTCGCATCTTTGGATGTTAACATCTACTATCATCTTTGCATTCAAAATAACAAAATTAAGCAAATGACACTTAATGGCAGTTGTTCATATGCATACATAATATCTCCTTCGTATTCATGACCTGTCATGTATAAAGCTGTCATTACTGATTTATGAACTCCCCGTTCAAGTACATTTTTACCAAGGTTTTCTTGGTTTTTATGTATAGTTCACGGACAAACAGCATATGGAAATTATCACATCTGTATGACAATGTCTGACCACTTTCTGCAG
Seq C2 exon
AGAGAGTACAAGAAAGCTTTGGAGCAAGAGATCAAGGGAAAAGGCATGTTGGCTTTGGCTACTGACACTCCAGACTTCTTGAGGGCAAGGAACGCCACAGAAATCCTCAGTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:124
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.196 A=NA C2=0.077
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=31.6),PF0088013=Nebulin=PU(55.2=42.1)
A:
NA
C2:
PF0088013=Nebulin=PD(41.4=30.8),PF0088013=Nebulin=PU(55.2=41.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]