RnoINT0099956 @ rn6
Intron Retention
Gene
ENSRNOG00000006783 | Neb
Description
nebulin [Source:RGD Symbol;Acc:1311134]
Coordinates
chr3:37681664-37682471:-
Coord C1 exon
chr3:37682361-37682471
Coord A exon
chr3:37681778-37682360
Coord C2 exon
chr3:37681664-37681777
Length
583 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
3' ss Seq
AACAAACAATTCTTCTACAGAAA
3' ss Score
6.16
Exon sequences
Seq C1 exon
AAAGAGTATAGGAAAGACCTAGAAGAAAGCATCCGGGGCAGAGGTCTCACTGAAATGGAAGATACTCCCGAAATGCTGAGAGCCAAGAATGCAACTCAAATCCTCAATGAG
Seq A exon
GTAAGACCGTTGTCAACTGCTGTGCGTATATAATTGATAATGCACCTTCATCCTACCCAAGAAAGACCGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAATCTTTCATTCTAAACTTTATATCCAGGCTAATTATTAACCGCTAAATAAAATAGGCATCCATTTCTGATGAATAGATATGAAAGCAGCATGGGCCTATAGAAGTTGTCATAACCAATTAGTGGCTAATATTACTTGACTTAGTTGTGATGTGTTCACAATGCTCATTCTAAAGGAAAACATCACACCACGCTGGGGTGGTTTGCACCTAAATTTAATCCTAATATGAACCTAACCAGATTTAAATTTTACTCTCTGTGCTAATGGGTTTCCCTTGGTTAACGTCACTGCACACGGAGCAGGAGGAACAAGCATGTCATACCGTCCACTGAAGGTAGCTGGCAAAGACAGCACCGGCAGCTACGGAGGAGCATGTCACGTGGACTTTTAACTCATGCAGCGCTGTCACCTACTAACAGAAGGCAGGGGACCTTTTTCTTTAAAACAAACAAACAATTCTTCTACAG
Seq C2 exon
AAAGAATATAAGCGAGACCTAGAGCTGGAAGTCAAAGGAAGAGGCCTGAATGCCATGGCCAACGAGACTCCAGATTTTATGCGGGCCAGGAATGCTACCGACATTGCCAGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000006783:ENSRNOT00000059461:96
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.838 A=NA C2=0.474
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(35.7=27.0),PF0088013=Nebulin=PU(55.2=43.2)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=28.9),PF0088013=Nebulin=PU(55.2=42.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAAGACCTAGAAGAAAGCATCCGG
R:
CTGGCTGGCAATGTCGGTAG
Band lengths:
214-797
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]