DreINT0103406 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23007696-23011031:-
Coord C1 exon
chr9:23010927-23011031
Coord A exon
chr9:23007801-23010926
Coord C2 exon
chr9:23007696-23007800
Length
3126 bp
Sequences
Splice sites
5' ss Seq
CAGGTATGA
5' ss Score
9.46
3' ss Seq
CTTTGCATTTAACCTAACAGAAA
3' ss Score
7.43
Exon sequences
Seq C1 exon
GCTAAATACAAGCAGAATGCTGAACATGACAGAGCCACATACACCACAGTCATTGACACCCCTGACATCCTCCACGCTCAGCAGATCAGGAATATCGTGAGCCAG
Seq A exon
GTATGACAGTCGCTTTTTTGAAAAAGGCTATTTTTACTTGTTTACTTGCTACGCTATGTAATGTAGACATAAAAGTTTCAAATCTAATTAAAGAATATAAAACAAAAACACACAATGGTTCATTTCCAAAGGTCAGCTAGCACCATTTTCCGCAACTACTAAACTAAGGTTTATGAACTTTATTAAAGGGGGGTCCACTCCGATATAATATTTTAAACTTTAATTGGTGTGTAATGTAGCTGTGTGAACATAAACAACATCTCTGGATGTAATATGCTCATATTTCAATGCAAAGGACCTTTGGGTACTACAAAAAAATATTTCCGGGCTATTGAGATCACAAGGGCTTTAGGTTACATGCATTTACTATGCCCATGTACCCTGTGCAGCGAAGGGGCGTGGCCAGAGGTGCTGTAATGTTATAGCAGATAAAGCTAAAATGCCGTCCAAACAATGCTATTTCCACAGAGCTTGTTCTGTTTCTGTATTTTGGCTGCCAAAAGACACAACATGAAGAGAGTAGTGCTTACAATTTAATTTTAGTTATTTTCCAGAGAATTATAAAAAATATATAGCTAGCATTTGACAAAGGAGAGCTTCTAGAATCTCTCTCAGTTTCGTGCTGAAATTGGCTAAAAACTCCTCAAAAGAGCAGTTCCAAAAAGCAGAAGCTGTGGATTATGAGCCACAAGCTGTAAGTATTTCTATTTGTTGGGGAATGCTGTTTGGCACCGCTAAAAATTTAGCTACAAATTCATATTTATCAGTCAAACCGCTGTAAACCCACAATCTTCACCAGCACTGCAGTGTCTCTCTATACGTCCACTTTTCATGCATTCTACATCTCAGATAATGATAAATACAGGATAGCTCACCTAACTCGTGTTGCAGCAGAAAAATCAATCAAAGCTTACATAGGTCGCATCCCACATCTTGCTCTTTATTCTCCTGTCAACAGCGTCACAGTATATTACAGAAAAATAACTTAATTCGAAAAATAAACATCGGTCCTGCACACACGCGGTTCTCATGTTACATGCGTCTACAGACTGTTCACACACACATACATAAAATGGCGATTCTCTCTTGAAGGAGCCACACCCTATTTTCACTCGTTACTGACATTTGTCAGATTATATTTTAGAGAGCAGGCATGAGGTTCATCCTTTTCATTTTCTGTCTGATTAAGGCTCAAACAAGATACTGCTAACAGCTACTCTGATTACCACTATTTACACAGCAGAATCAAAGCGCGTGCTAGTGACTGGACATAACGCTTTACTGATGACGTGGAGACGATCCGCTAATCACAGCACATTATGTTAGCTGACCAATCAGAGCATATTGAGGGCAGGCCTTTTGAATGAACTAGGAAATATGACAGTCGTTTTCATATTAGCAGAGTAGCTGTATATAATTAAAGTAAGATATATATAAGAAAATTGTGATTTTCTACAAATAAAGCATGAACACACATTGATTTGCATCTTATAAAGATAACCAAGCCTTAAAATTATACTCTTGACCACCCCTTTAAAACATGATGTTAAATGTATTATTCAATGGATAGTTTGCCCAAATATTAAAATCTGTCTTCATATACAGATTTTCGACTTGTTATAAAAATATTTGAGTTATTTTCCTCTGATGAACAGAAAATGTTTTTAAAAAAAAGCTAGAAACTGGTAGTCATTGACTACCAAGTTCCAAGGTATTTTTCTTACTATATATGCCAATAGCTACCATTTTTCAAAATGTCTTATTTAGTGTTCAGTGGAAAAATAAGGTGCTGGAACCACATAAGGGAGAGTGAATGGTGGGTTAACTATCCTCTAAGACAGGAGTTCTCAACCTTTTTCACTTTGGGGCCAACTTATTTCTCCGATGAATTTTTGAAGACCCACCTGTCTGACATTTTCTGTAAAATGTTTGTTTGAAATGGCCATTTAAATGAATGTAACTTACAGTGTTCTAGTTGCAGACACGATCATGAGGCTATTTTTGCACCGAGCTTGAAGTAATTAATCAGAGAACAGGAGAATGTGCATTACTTCAATCATTTTGATATAAATGATGAGATCAAAAAAATATAGTATAATAATGAGAGTTCATTTTAAGCTGTCTCGCTGCCCACCTGCAGTTTTGCTGAGGCCCACTGGTGGTCTGCAGCCCACCAGTTGAGAATCCTTGCTCTAAGGGAAATATTTAGTTTGACATTATTACAAAATACACCTAAAAACAGTACTTAAGGGTGTTAAGATATTGTCATGAAAGTGAACTCTCTTTAAGGACAATTTAAGTCTTAAATGTATTTAAATATACTTTATTATATTTAATTTGTTTTAAATATTTAATTGAGGTACAGTATTCAGCATAATTGCATACACCCCATTTTGAAAATTAATATTTTTATCCATTTCTCAGTGAGAAATGATTTATTAAACATATTTATTAAACAGATATATTTATTAAAATAATATTTTAGTCACCAAACATATTTAGATATTGAAAGAGAATACAATCAAACAAATTACAACCTACAAAATTTCAACTAAAATGTAAAAAAAAAAAATTGCTTTTTTTTAATCTGTATTTAATATTTTTCTATAAAATATAAATTTGGCTGTACTAGTTTTTGTACTGTTATCATGTTATTTTGTTAGACATGCTCCAGATTTGGCTTCTGTACTGACTAATCTAATGTATATGCACAAATATCATATTGTATAGCTTCCTATTAAAAATATGAATTTTAAAAGATAGATTTGTGAGAGGTGTACTTATATATGGAGAGTTCAGATGCAAAAACCTCTAATTGCCGTCTGAAATTCTCTTCTAAAATAAGCATTTTTATCAGGCTCCTGTGTGAATGTTCTATAATATCACTTTTATGGCAAAAAATAAGTTCTTTTCCTGGTCTTTAAAGTGAAATATCTCAGCAAAAACAAAGGAGGCTGAAAAAAATGGAAATTTATGATGGAAATTGCAGATGGCACTTTTGCATCTGAAGTCTTCATACGCTGAACACTCTATATGAAAAGTGCACATTAATTTCACTTGTTTTTCACATGGTTTGGGCTGATTATCGAGCTTAAAGTATTGTCGACTTTGCATTTAACCTAACAG
Seq C2 exon
AAAAAATACAAGGAGGAGGCTGAGAAGACCATGTCTCACTATGTGCCTGTCCTGGACACCCCAGAAATGCAAAGAGTGCGTGAGAACCAGAAGAACTTCAGCACT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:126
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.418 A=NA C2=0.630
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(55.2=44.4)
A:
NA
C2:
PF0088013=Nebulin=PD(41.4=33.3),PF0088013=Nebulin=PU(64.0=44.4)
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
No suggested primer sequences
R:
No suggested primer sequences
Band lengths:
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]