DreINT0103493 @ danRer10
Intron Retention
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23030049-23031192:-
Coord C1 exon
chr9:23031088-23031192
Coord A exon
chr9:23030148-23031087
Coord C2 exon
chr9:23030049-23030147
Length
940 bp
Sequences
Splice sites
5' ss Seq
GATGTAGGT
5' ss Score
4.9
3' ss Seq
CATGTTATTATCGTTTGCAGCTG
3' ss Score
8.76
Exon sequences
Seq C1 exon
CTGTGCTATAAGGAGAAGTACTACAACACCAGAGGAACTCTTCTGCCCATGCCCATCACCCCAGAGCTGATGCACTGCTTCCACGTCAATGAGATCAACAGTGAT
Seq A exon
GTAGGTCAATGTACTAGGGTTGGACGATTTCGACCAATTTGGCATTGTATGATGTTTAATGTGAAACATTGCGCTGGACGATGGCATCATCATTGTAGGCAACCGTTAATTATTTATGAATAATTAATTCATAACTAATTAATTATTTGTAGCCTACCATTACACACAAATAACCAGCAGTCAATCACTTTTTCCACGAAACTCTGGCACAAATAGGCAGAGTGATCTGTGTCGTTATAATGGCGTCAACAAACTTGGTAAAAAAAGGTGCACTACCAACAGGAATTAACCAACAGTATCTGAGGTTTTCACTAATATTACAAAATACAAGCGTGAAAGCAAAAGATTGAAGCAGTGTACTGACGCTGTGACACATTACCTGATAGATTTAAAAGACCGAAGAGTTGTTAGAGACAAGATTTATTAAATATCACAAGATTAATTAAATATCACACGATTAATCGAATATAGTGAGATGCAATCCAGCGGTGCATCCTTTATAAACTGTCCGACGTGCTCTGCTCTCTGGGGTTTTGTGCTTAAAGCACCCCATACTGACTGCCTGGAGCTCAGACGTGCACATGCCAGAGTGTGTATGTGAGTGTGTTTGTGTGTGGTCACATGATGTGCATTTTTAGCAGTATAGTATGGATGGAGAGATGTTCAGAAATAGTAGATAAAACGCCAATGTGGGAGTGGCTCGTTTTCATTCTAAAGTAGTGGGTTGCTACTGCAACGGGAACGTGAGGGAGGATCTCGATGCTGGCTCAGAATTGTGATGTCTTTCCGCCATTGGCGATGGACGATAGCATCGTTGATCGACCCAACCTTAAAATGCACCCAATTTACTTAAATACATAACACAATATAAACTATGCACATGTAGCATTTATTACGTTAGTGTTTGTAGGTATATTAACCATGTTATTATCGTTTGCAG
Seq C2 exon
CTGAAATATAAAGAGGATCTGCATTGGCTGAGGGGCATTGGCTGCTTCATGGTTGACTCTCCTGAGATGGTTCGCATCCGTGAGATCACCAAGTTCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630:ENSDART00000143888:96
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=33.3)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCTATAAGGAGAAGTACTACAACACC
R:
GTGATCTCACGGATGCGAACC
Band lengths:
190-1130
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]