DreINT0103500 @ danRer10
Intron Retention
Gene
ENSDARG00000021200 | nebl
Description
nebulette [Source:ZFIN;Acc:ZDB-GENE-050320-151]
Coordinates
chr2:21422207-21423060:-
Coord C1 exon
chr2:21422953-21423060
Coord A exon
chr2:21422385-21422952
Coord C2 exon
chr2:21422207-21422384
Length
568 bp
Sequences
Splice sites
5' ss Seq
AATGTATGC
5' ss Score
3.91
3' ss Seq
CGTCTCTCTTCTTTTTACAGGCG
3' ss Score
11.48
Exon sequences
Seq C1 exon
GTGAAATACAGGAAGGATTTTGAACAGAGCAAGGGACGGGGCCTCAAATTTGTCCTGGATACTCCTGAGCTACAGAGACTGAGGAAGGCCCAGGATCAGATCAGTAAT
Seq A exon
GTATGCTTAATAATTCAGGAAAAAAAAAGATGTAGCTAAATGGAAGTGTTGGGGGGTGGGGGTGGGGGGATAAAATGAACATTTAATTACTTATTTAATTTATGTTTAATTAGTTAGCTGTGGTTTTTAGGTATTCAAACTGGAGCAGGGACCCACAGAGGGCCACAAGTTTTAGGGATCTGAGGAAAGTCTGGAGAAAAAGCATAAAAATTATGCAAATAAACATAATATAAATATATAAAAATGTAAAATAATAAATATTAAGATAAAAACTGATACATAAATTACATTAATGAATAATAAACAACTTGATTTATCACAATACAATACTAGAAAAGCGAGTAATGTCATACACACTTCATATTTTCAAAATAATATTGTAAAACAATATTCTTCCAAGCACAATGACAATGATGGTCTTCCCAAGAGTAGAAAGACTAATATTGTTAAATATTACATGTGAATACTGTAACATAAACTGAAATGTATTCAATTTCAACAGCATTAATCCTTCAGAAATCATACTAACATGTGCACGAAACGTGTGGCGTCTCTCTTCTTTTTACAG
Seq C2 exon
GCGAAATATCATAAGGACTTTGAGGTGTCAGGTCTGCAAGGGGTCACTCAGGGAATGCGTGGGACGTACGCGGTGCGATCTCAGATGACGCCGAACAATGAAACTGTGGCTAACAGCACACACAAGGCTGTACATCAGTACATTATGGAGATGGACCGAAGACCTGGTGTTATCGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000021200:ENSDART00000032502:4
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.194 A=NA C2=0.417
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(59.3=44.4)
A:
NA
C2:
PF0088013=Nebulin=PD(33.3=15.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTGAAATACAGGAAGGATTTTGAACA
R:
CCACGATAACACCAGGTCTTCG
Band lengths:
286-854
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]