DreINT0106209 @ danRer10
Intron Retention
Gene
ENSDARG00000009341 | nrap
Description
nebulin-related anchoring protein [Source:ZFIN;Acc:ZDB-GENE-060616-224]
Coordinates
chr12:30484776-30485099:+
Coord C1 exon
chr12:30484776-30484880
Coord A exon
chr12:30484881-30484991
Coord C2 exon
chr12:30484992-30485099
Length
111 bp
Sequences
Splice sites
5' ss Seq
GATGTAAGC
5' ss Score
6.16
3' ss Seq
GTCTTTCTCCACATTTACAGATC
3' ss Score
9.58
Exon sequences
Seq C1 exon
CTGAACTACAAAGCCAACTATGAGAAGACAAAGACTCAGTATACGTTATCTCAAGACATGCCAGAACTTAAGAAGGCCAAAGCCAATGCTGAACTCGTCAGTGAT
Seq A exon
GTAAGCCAATATTCATACGCTCCTCAGCCTTGGCACGGTATTTGTCATCCATTTATATAGTTCCCAGGTGTTGACTGGATTGTTAAGCGCTGTCTTTCTCCACATTTACAG
Seq C2 exon
ATCAAATATAAAGAGGACTGGGAGAAAAACAAATCCAAAGCATGTGAAATTGGCCTGGACACACTGACTCTCAAAGCTGCCAAAGCCTCTCGAGATCTGGCTAGTGAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009341:ENSDART00000124920:17
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.257 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
NA
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAACTACAAAGCCAACTATGAGA
R:
ACTAGCCAGATCTCGAGAGGC
Band lengths:
210-321
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]