DreINT0116806 @ danRer10
Intron Retention
Gene
ENSDARG00000040002 | pnpla7b
Description
patatin-like phospholipase domain containing 7b [Source:ZFIN;Acc:ZDB-GENE-050309-66]
Coordinates
chr5:24551926-24554100:-
Coord C1 exon
chr5:24554037-24554100
Coord A exon
chr5:24552095-24554036
Coord C2 exon
chr5:24551926-24552094
Length
1942 bp
Sequences
Splice sites
5' ss Seq
CAGGTAAGA
5' ss Score
10.77
3' ss Seq
CTATATTATTTTCTTCTCAGTGT
3' ss Score
7.43
Exon sequences
Seq C1 exon
GTCCCACTTTGCTTCTAACCAGTGACATTATCAGACAGCGCCTGGGTGTTGCTGCCTTGGACAG
Seq A exon
GTAAGAGATGCATACAGTGCAAGAGATAAAAAAAAAAAACATATTACCTCACTGATAATTCGGACAAAATTATTTTTATTATGTTCAGGCATAGAAATAAATAAAGAGATCACTTGAGAGACCAACTCAAGTGTGAGACTTATTAAAAGTGGGTTTGAGTAAAAAAATGTAAATTTGCTGTATTCTATCAAAAAAATAAATAAATAAATAAAACAATTATTAAAAAAAATTATTATTATTATTAATATATAATATAATTATTATTAATATATATTATATATATATATATATATATATATATATATTTATATATATATATATATAACTATTATGAATTATATATAATTTTATATATTTTAAAACAATCTAAAATATAAAATCAAATATCCCCAAAAAATAATTATTTATTATTTATTATTTATTATATATATATCGGCACTGGTGGGAGGTATACTACCAGTGTTCCACCAGTGATGAAATACAGCCACATTGCACTGCTACCCGTGTGATATTGCATTTATACAACAGTTCAACGACAATCATGTATATAAAACAAAAAATCAAACACGGAGAGTCTAAAAATCTTTTTGTATGAGGAACTACTTTCTTCAATCTTTCAGCCATTCCTTCACATCTGCGGCAAAAGACAGAACAGCAGAAGCTGTTGCTACTTCGCCAATGTCACTTTAGAGATAGTGTTTGAATGAATCTCTAGCATAATGTCTAAAGTGAAGATATTAGAATCTTAAGATTATAAGGCTGAATGGCATGAAATTAATATTTCAACGAAACAGGATATTTATAACGAAAACAGGAGGCTCATTTAAAACAAACAGATGGCCAAACAAAAAGTAACTCAGGGTTTTGAAAAGAGTGGCCAACACAAAATACATTCCTGAGAGTCCCATCTCACACTAAATACACTACAGTTATATGGTATAAATACAGCACTACAATATACAAAAGAGAGAGATCAACTTAAAAATGTCCCTTTCATGTTTAATAATGATTTAATCAGTTGTAGTTAGAAATTACGCTGTTTCATCTTCTAAGAGCTTTAATTAAATTAAATTAAACCTAAATCACACTGAACTAAACTTCAACTGAACTGAAATCTGGACTTTACTAGAATTGTTAAGCTGCTTTGACACACTATACATTGTGAAAAGTGCTATATAAATAAACATGAATTAAATTGAATTATGCGTTCTCTATCGCCATTATGTGGATGGACAACGTCAACCATCTTTGCTCTGCTCAATGACAGGCTAATGACCACAGACGTCTCTCAGAAATTATAAATATTTAAAATAGGCACTATCCTTATGCATGCATATATAAATATGTATATTTTTGATTATTGATTTTATATTTTATATTTTATATATATATATATATATATATATATATATATATATATATATATATATATATACCATGAATACCATATTTATTGTATGCACTCTGTACACAGAATGTCAACACTAAGCTATGTTAACAAAAAGCTGAACGAAAAATGCAAGTAAGATAAATTTGCTCTATAAAACAAACACCATGTCCACAAACTCTAAACAATCCACAAATTTGAAAGACGAAAATAGTCTCTCATGTCTTTTCCAAAATTGAAAGATGCTGATTTCCATCATAATTAGGTGTGCTAGTATGGTTTCTGGGCCCCCTGGACAGTATAATAACCTCCTCAGTAGAAGTCAGCAAGAGAACTGCAAATAATTGTGGCCATCGCTGACTGAAAAAGATGCACAGTTTGTAAATACACTTACTGGAAACCATACATCGCCTTTGACATGCTGTTTATAAACTATGGTTGAATGTGTGCAAATTGGGAACCAGAGTATGCTTTTCCCAGTCACGTTAGTCAAACATTGTCTTTTGATCTCCTCTGATGTACTGTTTATTTGATTCTATATTATTTTCTTCTCAG
Seq C2 exon
TGTTCACGAGTATCGTTTGTCAAGCTGGCTGGGTCAACAAGAGGACATCCACCGCATTGTTTTATATCAATCTGATGGTTCTCTGACTCCATGGACTCAACGGTGCATTCGACAGGCAGACTGCATCATCATCGTGGGTCTGGGAGAACAAGAGCCCACTGTGGGAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000040002:ENSDART00000145061:22
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCCCACTTTGCTTCTAACCAGT
R:
CACAGTGGGCTCTTGTTCTCC
Band lengths:
227-2169
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]