DreINT0124823 @ danRer10
Intron Retention
Gene
ENSDARG00000089933 | rasal3
Description
RAS protein activator like 3 [Source:ZFIN;Acc:ZDB-GENE-101130-3]
Coordinates
chr6:8239900-8240784:-
Coord C1 exon
chr6:8240122-8240784
Coord A exon
chr6:8240045-8240121
Coord C2 exon
chr6:8239900-8240044
Length
77 bp
Sequences
Splice sites
5' ss Seq
AAGGTAATT
5' ss Score
8.83
3' ss Seq
TTTTTAATTTTTTTTTGCAGGAG
3' ss Score
11.29
Exon sequences
Seq C1 exon
GACAACTGTGAGCGCACTGAAAACTCACTGTGCCTTTGGATCAATGAAGCAAAAGACCTTCCCCCAAAGAAGAGATATTATTGTGAGGTCCACCTTGATGGTACACTGTTTGCTCGTACAAGTAGTCGGGCTGTAGGGAAGTCTGTCCAACGCTCCAGCCAGGCAGTTGATGGAGGATCTGGAGCGTTCGGCAGTGGTGGTACTTCAGGAGGTTGCCAGTTGTTCTGGGGTGAATTTTTTGAACTTGACAACTTGCCCCCTGTCACCCAGATCACCCTGCACCTCTTTAAAGGTGAAGACCCCAAGAAGAAACGGCATTCCAGGGACGACGCTACCCTGCATCCTTTAGGAAGTGTAGCTCTCCCTTTGGCAGACATAAAAGGACGTGCCTATCAAGAGAAATGGTATCGAATTGTTGCCTATAAACCCCCGGGAACAAGTACAGCAAAGGATTCGGTTGAACTGCAGGCTTCAATTCGAGTCAAGGCCAAATTCAAGAACCTGAAAATTCTACCTATTGAGAGGTACAAGGAATTTGCAGAGTATGTCACTGTGAATTATGTGGATATGTGTAGCAGCCTTGAACCCCTTCTCAATGTGCGGGAGAAAGAAGAACTAGCTGGAGCACTTGTTCATGTGCTCCAAAGCATTGGGAAAGCTAAG
Seq A exon
GTAATTAGCACAGCACAAATCGTAGGACTGGGCAGAGTTCTTCAAATTTCTAAACATTTTTTAATTTTTTTTTGCAG
Seq C2 exon
GAGTTCCTGATTGATCTTGGTAGTGCAGAAGTTCAACGCTTGGGAGAAAATGAGGCTTTGATTTTCAGGGAGAATACTCTGGCTACAAAAGCTATTGATGAATATATGAAATTGGTGGGCCAGAAGTATCTCATTGACACACTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000089933:ENSDART00000124643:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.086 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0061614=RasGAP=PU(27.3=95.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCTGGAGCACTTGTTCATGT
R:
TGAGATACTTCTGGCCCACCA
Band lengths:
179-256
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]