DreINT0145826 @ danRer10
Intron Retention
Gene
ENSDARG00000063444 | si:dkey-32n7.7
Description
si:dkey-32n7.7 [Source:ZFIN;Acc:ZDB-GENE-121214-179]
Coordinates
chr12:4148288-4148789:-
Coord C1 exon
chr12:4148666-4148789
Coord A exon
chr12:4148567-4148665
Coord C2 exon
chr12:4148288-4148566
Length
99 bp
Sequences
Splice sites
5' ss Seq
TTTGTAAGA
5' ss Score
3.14
3' ss Seq
AATGTATTTTGATCCTACAGGTG
3' ss Score
9.86
Exon sequences
Seq C1 exon
AATATTTTATCCATATGGGGATGCAGAGGATGAGATAAACCCACAGTCAGATGATGGAAGTTCTCCACCAATCCCCTTGCTGCAGTCATTTGTGTACTTTGGAAATACATATGACACGATATTT
Seq A exon
GTAAGAAGCATTCATTAACGACATTCTTATATTTTCCATCTATGATGTTGAATTGTGGTGATAATTAAAAAATTGAGGTAATGTATTTTGATCCTACAG
Seq C2 exon
GTGAATAATAATGGAGACCTGACCTTTGATAAACCGCTTCACCAGTATAATCCTGATAATTTTCCTGCATACTCAACTAGAGATATCATCGCCCCTCTGTGGACAGATATTAATAATGGAATGGAAGGAACTATTTCGTACCGACAAGTAACAAATGGTGATATCTTGAATCGGGCGTCAAAAGACATTAACAGATATTTTCCCAACTTAAACTTCTCTGCCTCATGGGTTTTCATTGCTACATGGGATAAAGTACCGTACTATGGATACAGAGAGTCA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063444:ENSDART00000152313:1
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.310 A=NA C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
NO
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTCTCCACCAATCCCCTTGC
R:
GTCCACAGAGGGGCGATGATA
Band lengths:
169-268
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]