DreINT0146879 @ danRer10
Intron Retention
Gene
ENSDARG00000086494 | si:dkey-7i4.2
Description
si:dkey-7i4.2 [Source:ZFIN;Acc:ZDB-GENE-131121-32]
Coordinates
chr15:821847-823846:+
Coord C1 exon
chr15:821847-821968
Coord A exon
chr15:821969-823171
Coord C2 exon
chr15:823172-823846
Length
1203 bp
Sequences
Splice sites
5' ss Seq
CAGGTCAGT
5' ss Score
8.94
3' ss Seq
ATATGTATCTTGTATGTTAGACA
3' ss Score
4.48
Exon sequences
Seq C1 exon
AGTGTCAAAACCCACAGAACAGCAGATTATGATGAAGATGGCGTTTATTAAAGAGGAGAGTGAAGACCTGAGGATTGAAGAAACATTCACAGTCAAACATGAAGAGACTGAAGAACAAACAG
Seq A exon
GTCAGTTTTCATCCACAACTATATGCAAATGTCCAGCTCTTCCGAAATTAATAATGGACAGTACTTTCTGTGATCTTATACTCGATCCAAGGGTCAGTTTTGGTCGGTTATTAGTGACTATTTAACATTTAAACAAGATGCAAATAAACTAAACTGAACTTCAGCTCTGAAATCTGGACTTTACTAGAACTTCTATGTTAAGCTGCTTTGACACAACCTACATTGTAAAAAGCGCTAAATAAATAAACATGAATTGAACTTTTTAGTATGTAATGTTCATCTAGGGCTGGGGTTTTTTTTTCCGATTAATTCGAATTTACGTTTTGATGACGATTTAATTTTATATTAAATCGAGAAATTGCGATTTAAAAAAAAATATATATTTAATACATTATAATGGCACCAATGTGCTTTGTTTCACTCTCTGTATGAAGCAGGAAGCACACCAGAAGCGCCTCTCGGCGACGCGCCACGCAGCGCCATGGATTTTACAATTCTAAACAGATTTCTATACACACACCGGCGCCGGCGAGCCACGATTCAGGGTGCAGTATATATTGCAGCCGCGCCACAGAGCGCCATCTGATTAGTTTCATGTTCAATATCATGCGAATGTGCGCGTTTGGTGTGTGATAGTTTAAACTGTCATGTGCGTGCCGTGTCGCGTCGCTTTGTGCGACCTGCTTGATTGTCTGTTAAAGCGTGAAGACAGGTAGGATTTTACTTGTTGCATTAGTCTGAGTCACGTCTGGATTGTCAAGACATATTCCCTCATCAAGTCGATAAACTCGATCTCAACATGTATGAAGGACGTAAAACCCTGCCATGTGAAAAACCACACCGATCTTTCGGTTTGAACACGAGCAGAGGTGAGGGTCTGTAGCAGTGAGAATGAAAGTAGCCGCCCCCATGATTTAGCGGACCTGGTTGAAAACCAGACAGATCAGGCAGCATAATACAGAGAGTGGAGCAAAGTGCGTCAAATGATCGATATTTAAAAGGGGGAAAAATAAAAATAGCTTTAATATATTATTTTATATTAGACACACATCTGGTGCTTTTATTTGCTCCTGCTATACATCGACAGCTTCTGGGATTCTGGGATTGTAAGAAACGGCAGTTTCAGCAGCATGTTTTGAATCTCTATAAATTTTCTTTCATTTAACTTTCATGTATCTGTCTTATATGTATCTTGTATGTTAG
Seq C2 exon
ACATGATGGCATTCAAAGAAGAGAATCAAGAACCGAAGAACACTGAAGAACTTGATTCGAGAGAGGAACATGCGAGACTTCACGCTGCAGAAAAGCCACACACGTGCCCTCAGTGTGGAAAGAGTTTCAAACAAAAAGGAAAACTTCATGTCCACATGAGAATTCACACCAGAGAAAAGCCCCTCAGCTGCAGTGAATGTGGACTAAACTTCAGTCGAAAATCAGTCCTGAGCAGACACATGAGGACTCACTCTGGAGAAAACCCCTTCACCTGTCGGGAGTGTGGGATAAGCTTCAGTCGTAAAGATGCGTTGAGCAGACACATGAGGGTTCACACCAGAGAGAAGCCTTTCAGCTGTCAACAATGTGGAAAGAGTTTCACTCAAGATGGAAGCCTCAAACGCCACATGCTAACACACACCGGAAAGAAGACTCACACCTGCCAGCAATGCGGACAACGGTTTTTCAATAAATGGATTCTTGAACGCCACATGAGACAGCACACAGGAGAGAAGCCTTACACATGCCAACAGTGCGGAAGAAGTTTTACTCTTAAATCAACTCTTGATGCGCACTTGAGGATTCACACCGGAGAGAAGCCGTTTGTCTGCAGCCAATGTGGGAAGAGTTTCAGACATAACGTGACCCTGACTAACCACATGAAGACTCACAAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000086494:ENSDART00000154903:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.838 A=NA C2=0.322
Domain overlap (PFAM):
C1:
NO
A:
NA
C2:
PF0009621=zf-C2H2=WD(100=10.2),PF132401=zinc_ribbon_2=WD(100=16.4),PF134651=zf-H2C2_2=WD(100=11.5),PF134651=zf-H2C2_2=WD(100=11.5),PF134651=zf-H2C2_2=WD(100=11.5),PF134651=zf-H2C2_2=WD(100=10.6),PF134651=zf-H2C2_2=WD(100=11.5)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGAGGAGAGTGAAGACCTGAGG
R:
CCGACAGGTGAAGGGGTTTTC
Band lengths:
349-1552
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]