DreINT0150013 @ danRer10
Intron Retention
Gene
ENSDARG00000035468 | slc25a25b
Description
solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 25b [Source:ZFIN;Acc:ZDB-GENE-060526-340]
Coordinates
chr5:31204471-31204883:+
Coord C1 exon
chr5:31204471-31204578
Coord A exon
chr5:31204579-31204715
Coord C2 exon
chr5:31204716-31204883
Length
137 bp
Sequences
Splice sites
5' ss Seq
GAGGTAAGA
5' ss Score
10.06
3' ss Seq
AAAGCTTCTGTGTGTTTAAGGTT
3' ss Score
7.99
Exon sequences
Seq C1 exon
ATTAAGCGGCTCATTGGCAGTAATCAGGAGACGCTGGGGATCCTAGAGAGACTGGTTTCTGGTTCTCTAGCAGGCGCAATTGCGCAGAGCAGCATTTACCCAATGGAG
Seq A exon
GTAAGAACATAAACTTTATTACTGTACATTTCAGTAACTCACCACCATCTTTTTAATTATGTTCTCCTTTCTGCATCAAACAAAGCATGACTTTGTTAGATGCCTCAGAGCTTAAACAAAGCTTCTGTGTGTTTAAG
Seq C2 exon
GTTTTAAAGACTCGTCTTGCTCTGGGAAGGACGGGTCAGTACTCGGGAATTGCAGACTGTGCTAAACATATTTTTAAGAAGGAGGGCATGACAGCTTTCTATAAGGGTTACATTCCCAATATGCTGGGTATCATCCCTTATGCTGGAATAGACCTGGCTGTTTATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035468:ENSDART00000098163:7
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0015322=Mito_carr=PD(8.5=22.2),PF0015322=Mito_carr=PU(28.0=72.2)
A:
NA
C2:
PF0015322=Mito_carr=FE(59.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGACGCTGGGGATCCTAGAG
R:
ACAGCCAGGTCTATTCCAGCA
Band lengths:
242-379
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]