DreINT0169767 @ danRer10
Intron Retention
Gene
ENSDARG00000037997 | tubb5
Description
tubulin, beta 5 [Source:ZFIN;Acc:ZDB-GENE-031110-4]
Coordinates
chr16:10432913-10436679:+
Coord C1 exon
chr16:10432913-10433023
Coord A exon
chr16:10433024-10434777
Coord C2 exon
chr16:10434778-10436679
Length
1754 bp
Sequences
Splice sites
5' ss Seq
TTGGTGAGT
5' ss Score
9.27
3' ss Seq
TCCTCTTTGTCTCCTCACAGGAC
3' ss Score
12.39
Exon sequences
Seq C1 exon
GAGGTAAATATGTGCCAAGAGCCATTCTAGTGGATCTGGAGCCTGGTACCATGGACTCTGTGCGTTCAGGGCCCTTTGGACAGATCTTCAGACCTGACAACTTTGTATTTG
Seq A exon
GTGAGTGACTGATAACAAGTCTATCATCACACAAAAAATCTAATTGACAAAAATTATGGAACAGAATCACAGGGTTATCTTACCCAAAAACACAATTATTTTACTAATTATTCACCCTCATGTCATTCCAAGCCCCTGAGATCTTTGTTCATCTTCAGGACACAAGTTTTGATTTTAAATTAAGGTGAAATCTGAGAGCTCATTCATCTTCCATAGACATCAATGTTCCTCGCTTGTTTAAAGTCAAGAAAGGGACCAAAACTTTCTCAAAACATACCATATGTCTTCAAAGCTTCAATCAGAATATTATAGAACTAAAAGAATACTTTTGTGCACATATAAAACAAAAACAAGGACTTTATTCCATGGCTTCTTCTGTTAAGTGTCAGTATATTGCACACATCTTTAAGTACTGTGTGGTGACGAATATCCCTCTGATGCCTGTGATTGGTTTCCTCTGTTTGAAACAAAGCACTGGTGCATCCGAGGTAATATGAATGTGACACCTGACACATGAATGTGCGCCCTGTATTGAATGATTTTTCTAACTTCTTCTGGAAGAGTCTGGAACATTGCTGTATATAGAGAATAAGGAAGCCCTCAGATTTAATCTAAAACATAATTATCATAGCCATATCTACCTGCTGCTCAAGACCGGTTACTCACTGAAGCTAAGCAAGGCTGGGCCTGGTCAGTACCTGGATGGGAGACCCAATGGGAAAACTAGGTTTCTGTTGGAAGTGGTGTTAGTGAGGCCAACAGGGTGTGGTCTGTGCGCTCAATCTGTGGTCTGTGTCCTAATGCCCCAGTATATTGAAGGGGACACCATAACGTTCAGTGAGCGCCGTCTTTTGGATGAGACGTTAAACTGAGGTCCTGACTCTTTCTGGCCATTAAAAATCCCATGGCACTTCTTGTAAAGAGTAGGGGTGTAACCACAGTGCCCTGGCCAAATTCCTTCCATCGACCCTTACCCAATCATCCCCATCCACCAAATTAGCTCTATCACTGTCTCTCCACTCCACCAATAGCTAGTGTGTGGTGAGCGCACTGGCGCCGTTGTCTTGTGGCTGCCGCCCTGTCATCCAAGTGGATGCTGCACACTGGTGGTGGTGTGGAGAGACCCCCCCCCCTCATGATTGTGAAGCGCTTTGGGTGTATGGCCACACACAAATGCGGTATATAAATGCATGCTACATTACACCATTAATTTATTCGACTTAGTACCTGATTTATCAGGGGTTGCCACAGTGGAATGATCCGCTAACTGCTCTGGCATATGTTTTACGCTGCAAATGCCCTTCCAGCCACAACCGAGTACTGAGAAATGCACATGCACCCTCGCATTCACACGTGCACTTATACACAACAGCCGATTTAGTTGGTGTGTGATGGAAACCGGAATACATGGGGAAAACCCACGTGAACACGGGGAGAATATGCAAGCTCCACACAGAAATGCCAACTGACCCAGCCGGGATTCGAACCAGCAACCTTATTGTTGTGAGATAACAGTGCTAACCACTGAGCCACCATGGTGCCTTAAAATATAATAGAATTATTTTGTATTCCAAAGACAAAGATAGATCTCAGGGGTTTGGAGCCTCATTAGTAGTCGTGGTATATATTAAATAAAAGAATTTTCATTTTAGGCTAAACTAACACTTTAAAAGATCCAATTAAAGCAGGAAAAAGAAAAATGCACCTCATCCTGCACAGTCTGAAGTGTGTTCTTCCTCTTTGTCTCCTCACAG
Seq C2 exon
GACAGAGCGGTGCTGGTAATAACTGGGCCAAGGGCCACTACACGGAAGGTGCTGAGCTTGTAGATTCAGTTCTGGATGTGGTCCGCAAGGAGTCCGAGAGCTGTGACTGCCTGCAGGGCTTCCAGCTCACTCACTCCCTCGGCGGTGGAACCGGCTCCGGCATGGGTACCCTGCTGATCAGCAAAATCCGCGAAGAGTACCCAGACCGCATCATGAACACATTCAGCGTGGTGCCTTCGCCAAAAGTCTCTGACACCGTAGTGGAGCCTTACAACGCCACGCTGTCGGTTCATCAGCTGGTGGAGAACACAGACGAGACCTACTGCATAGACAATGAAGCCTTATATGATATCTGCTTCCGCACCCTCAAACTCACCACCCCCACATATGGCGACCTCAACCATCTTGTGTCCGCCACCATGAGTGGTGTCACCACCTGCCTGCGCTTCCCCGGCCAGCTCAACGCCGACCTTCGCAAGTTAGCCGTCAACATGGTGCCATTCCCCCGTCTGCACTTCTTCATGCCCGGCTTCGCTCCTCTGACAAGCCGAGGCAGCCAGCAGTACCGCGCCCTCACCGTGCCTGAGCTCACGCAGCAAGTTTTCGACGCCAAGAACATGATGGCGGCCTGCGACCCAAGACATGGGCGCTACCTGACCGTCGCCGCTGTCTTCCGAGGCCGCATGTCTATGAAGGAAGTGGACGAGCAGATGCTGAACGTCCAGAACAAGAACAGCAGCTACTTCGTGGAATGGATCCCCAATAACGTTAAAACCGCCGTCTGCGATATTCCACCTAGGGGTCTCAAAATGGCCGTCACCTTTATTGGTAACAGCACGGCCATCCAAGAGCTGTTCAAGCGTATCTCCGAGCAGTTCACGGCTATGTTCAGGCGTAAGGCTTTCCTGCACTGGTACACGGGCGAGGGGATGGACGAGATGGAGTTCACCGAGGCCGAGAGCAACATGAATGACCTGGTGTCCGAGTACCAGCAGTACCAGGACGCCACCGCCGAGGAAGAGGGCGAATTCGAGGAAGAAGCCGAGGATGATGCTTAAGAAGGAAACTAAAGGGTGTTTTGGGGAGGTGGTAGTGTGTTTAAGTTTTGAGTAAATGTAGCAAAACTTTTATGGAAGTGTCTCAATCAGATGAAGTAGAAATTAGCACTAGACAAAAAGGGGCCAAAAATGAACAGAGGAAGGCAGAAAGAATGTTTTTTAAACATGGATTAGAGTGGGTAACAGTGTTTACGATTACAGTTCACTCTTTTTAGTGTGTATGAGTGTATTAGTTTACCATTCTGATGGTGCGGTACAGAACAGGGAAGCGAAAAGTGGCAAACATCCAGGTAGAATCAGTGTTACAAATTGGGAGGACTGTAACATCTCATTTAAGTTTTATTTGAACGTTTGCTTCTTTTATCTACAAGTAACTTCAGTGAAATTTATCGTGTTTTATTTGTGTGATTTCTTTTGTTACTATTGCACTAGAACAGGGCAGCTCTGTGAAATAATGGCACTTCAATGTGACCTCCAGGGGTCTGATTGCAGTATTGCAAGAAATGTTTGGAAAAGATAAGCATGTCATGGAAATGGTTATGGAAAAGGACTGATGGAATGACAGGGTTATTGAATCTTGGCCTCACTTGGACGCCAACCAACGTCTACAGGAGGACATAAATCAATGGATGGCAAGGGCAATACAGGAAAGGGAAGTATTTTAGATTAGGCAAATAGAAAGACAAGCATGTTGAATGGATTGTTGGTGCAATTTTTTAATTCATGGACTCTGTTATGTCTTATTTCTGAGCCCATCGTTTTAAGTGTTATATTGTTGATTATATATTTCAACAGTTTTGATGTTCCTTGATTGTCCCTGTGTGGAAGGAAACTGAGCCTCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000037997:ENSDART00000055380:3
Average complexity
IR
Mappability confidence:
NA
Protein Impact
Alternative protein isoforms
No structure available
Features
Disorder rate (Iupred):
C1=0.026 A=NA C2=0.097
Domain overlap (PFAM):
C1:
PF0009120=Tubulin=FE(16.7=100)
A:
NA
C2:
PF0009120=Tubulin=PD(59.0=37.1),PF0395312=Tubulin_C=WD(100=34.8)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAGCCTGGTACCATGGACTCT
R:
GGCGTTGTAAGGCTCCACTAC
Band lengths:
351-2105
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]