GgaALTD1022810-2/3 @ galGal4
Alternative 5'ss
Gene
ENSGALG00000013370 | Sema3F
Description
semaphorin-3F precursor [Source:RefSeq peptide;Acc:NP_989589]
Coordinates
chr12:3183997-3184954:+
Coord C1 exon
chr12:3183997-3184173
Coord A exon
chr12:3184174-3184200
Coord C2 exon
chr12:3184866-3184954
Length
27 bp
Sequences
Splice sites
5' ss Seq
GAGGTGCTT
5' ss Score
3.62
3' ss Seq
GGCACCCTGGTGTCTTGCAGATC
3' ss Score
8.58
Exon sequences
Seq C1 exon
TGCCCCGGGGGGACCTTCACACCCTCCATGAAGTCAACCAAGGACTACCCCGACGAAGTGATCAACTTCATGCGCTCTCACCCTCTGATGTACCACGCCGTCTACCCGGCCCACCGGCAGCCTCTGGTCGTGCGCACCAACGTCAACTACCGCTTCACCACCATCGCTGTTGACCAG
Seq A exon
GTGGACGCGGCAGATGGGCGCTATGAG
Seq C2 exon
ATCGGGGCACGGTGCAGAAAGTCATTGTGCTGCCCCGGGACGACATGGAGACAGAGGAGCTGATGCTGGAGGAGATCGAGGTGTTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000013370-21-26,22-26,23-26-2/3
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact
ORF disruption when splice site is used (sequence inclusion)
No structure available
Features
Disorder rate (Iupred):
C1=0.147 A=NA C2=0.067
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(16.7=100)
A:
NA
C2:
PF0140314=Sema=FE(6.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Conservation
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AACGTCAACTACCGCTTCACC
R:
CCTCGATCTCCTCCAGCATCA
Band lengths:
120-147
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]