GgaEX1065945 @ galGal3
Exon Skipping
Gene
ENSGALG00000003135 | Q90717_CHICK
Description
NA
Coordinates
chr6:10108886-10124456:+
Coord C1 exon
chr6:10108886-10108988
Coord A exon
chr6:10116575-10116681
Coord C2 exon
chr6:10124232-10124456
Length
107 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTTTTGTTTTCTAAGCAC
3' ss Score
6.64
5' ss Seq
TGGGTAGGT
5' ss Score
6.86
Exon sequences
Seq C1 exon
CCTCCGCTCCTTCAGTTCGGATAGGTCCTACACTCTGAACAGAAGTTCTTATGCCCGAGACAGCATGATGATTGAAGAGCTGCTGGTACCAGCGAAGGATCAG
Seq A exon
CACCTGACGTTTCAGAGGGAGTTTGATTCTGATTCTCTCAGACACTACAGCTGGGCTGCAGACACCTTGGACAATGTTAACCTTGTTTCAAGTCCCATCCATTCTGG
Seq C2 exon
TTTCCTGGTTAGTTTTATGGTCGATGCACGTGGGGGTTCGATGAGAGGAAGTCGTCATCATGGAATGAGAATTATCATCCCACCACGCAAATGCACAGCGCCAACCCGCATCACCTGCCGCTTGGTAAAGAGGCATAAGCTGGCCAGCCCACCACCAATGGTTGAAGGAGAAGGATTAGCAAGTAGACTTGTAGAAATGGGGCCGGCAGGGGCACAATTCTTAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003135-'32-33,'32-31,33-33=AN
Average complexity
A_C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.180
Domain overlap (PFAM):
C1:
NO
A:
PF0079115=ZU5=PU(1.0=2.8)
C2:
PF0079115=ZU5=FE(91.5=100),PF0079115=ZU5=PU(0.1=0.0)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Cow
(bosTau6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCGAGACAGCATGATGATT
R:
CCGGCCCCATTTCTACAAGTC
Band lengths:
257-364
Functional annotations
There are 1 annotated functions for this event
PMID: 26024478
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926), exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]