DmeEX0000197 @ dm6
Exon Skipping
Gene
FBgn0011747 | Ank
Description
The gene Ankyrin is referred to in FlyBase by the symbol DmelAnk (CG1651, FBgn0011747). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (1 unique). Gene sequence location is 4:115074..129849. Its molecular function is described by: cytoskeletal anchor activity; spectrin binding; ion channel binding. It is involved in the biological process described with: signal transduction; protein localization to plasma membrane. 10 alleles are reported. The phenotype of these alleles manifest in: ovariole. The phenotypic class of alleles includes: viable. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period.
Coordinates
chr4:118555-120212:-
Coord C1 exon
chr4:119888-120212
Coord A exon
chr4:118838-118931
Coord C2 exon
chr4:118555-118779
Length
94 bp
Sequences
Splice sites
3' ss Seq
ACTTATTTTATTCATTCAAGAAA
3' ss Score
4.23
5' ss Seq
AGGGTAAGC
5' ss Score
9.04
Exon sequences
Seq C1 exon
GACGGGAACACAGCTCTCCACATTGCAAGTAACCTTGGATACGTAACTGTAATGGAATCCCTCAAAATTGTCACTTCAACATCCGTTATTAATTCAAATATTGGTGCGATTGAAGAAAAACTCAAGGTAATGACTCCAGAGCTTATGCAAGAAACCTTACTTTCCGATTCCGATGACGAATCTTGTGATGATCTTTTGGATCATAACCATTACAAATACATGGCAACTGATGATTTAAAAGCCAACTACGGCCAGGATCAGAAGAACTTCGATACTACAAACACCGACCACGATCTTACTGATGTTTCAGTACTAAATAAGAAAG
Seq A exon
AAATACTACCCAATGAAATGAGCTGTATTGAATTGACTGAAATCGGGCACAAACCCGATAACGTTGTTATTGCCCGATCCCAAGTTCATTTAGG
Seq C2 exon
GTTTCTGGTGTCGTTCTTGGTAGATGCACGTGGCGGATCCATGCGCGGATATCGGCACAATGGTGTGCGCATTATCGTACCTCCAAAGGCCTGCGCTGAACCAACACGAATAACATGTCGATATGTTAAGCCGCAAAGGGTAGTCAACCCTCCGCCTTTAATGGAGGGTGAAGCACTTGTGAGTCGCATTTTGGAAATGTCACCTGTTGACGGAATGTTTTTAAG
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0011747-'17-16,'17-15,19-16
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.174 A=0.000 C2=0.013
Domain overlap (PFAM):
C1:
PF127962=Ank_2=PD(28.7=22.9)
A:
PF0079115=ZU5=PU(1.0=3.1)
C2:
PF0079115=ZU5=FE(71.4=100)
Main Inclusion Isoform:
FBpp0088238
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
FBpp0088239, FBpp0088240, FBpp0289724
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
CCAACTACGGCCAGGATCAGA
R:
GGAGGTACGATAATGCGCACA
Band lengths:
168-262
Functional annotations
There are 1 annotated functions for this event
PMID: 26024478
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926), exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)