DreEX0013107 @ danRer10
Exon Skipping
Gene
ENSDARG00000061736 | ank3a
Description
ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]
Coordinates
chr17:20714204-20731587:-
Coord C1 exon
chr17:20731485-20731587
Coord A exon
chr17:20725276-20725385
Coord C2 exon
chr17:20714204-20714428
Length
110 bp
Sequences
Splice sites
3' ss Seq
GTGTTTGGGTGTGTTTACAGCAT
3' ss Score
7.26
5' ss Seq
TGGGTAAGA
5' ss Score
8.91
Exon sequences
Seq C1 exon
TCTGCGCTCCTTCAGCTCAGACAGGTCCAACACACTGAACAGAAGCTCCTTCGCTCGGGACAGCATGATGATTGAAGAGATTCTGGCACCCACCAAAGACACG
Seq A exon
CATCTTGCAGTGACCCGTGAATACAATAATGAGTGTATGCGGAGGTACAGCTGGACACCTGACACCGTGGACCAATCACATAACACTGTGTGCAGTCCTGTGCACTCTGG
Seq C2 exon
GTTCCTAGTGAGTTTTATGGTGGATGCTCGGGGCGGGTCGATGAGAGGAAGTCGTCACGATGGTATGCGCATAATCGTTCCACCCAGAAAGTGTCCAGCACCGACACGAATCACCTGTCGTTTAGCAAAGCGCCACCGCCTGGCATACCCACCACCTATGGTGGAGGGAGAGGGTCTTGTGAGCAGACTGGTGGAAATGGGGCCAGCTGGGGCACAGTTTTTAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061736-'34-37,'34-34,35-37
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.455 A=0.270 C2=0.171
Domain overlap (PFAM):
C1:
NO
A:
PF0079115=ZU5=PU(1.0=2.7)
C2:
PF0079115=ZU5=FE(71.4=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Primers PCR
Suggestions for RT-PCR validation
F:
GCTCGGGACAGCATGATGATT
R:
TCCACCAGTCTGCTCACAAGA
Band lengths:
246-356
Functional annotations
There are 1 annotated functions for this event
PMID: 26024478
The paper describes and annotates all exons to an extreme detail. Wild-type isoform, but not the variants (either skipping 31 or 28-31), bind beta-spectrin. Exon 28 (MmuEX0004926), exon 29 (MmuEX6048924), exon 31 (MmuEX6048923)
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]