GgaEX1066070 @ galGal4
Exon Skipping
Gene
ENSGALG00000003578 | fn1
Description
fibronectin precursor [Source:RefSeq peptide;Acc:NP_001185641]
Coordinates
chr7:4338162-4339311:-
Coord C1 exon
chr7:4339212-4339311
Coord A exon
chr7:4338845-4339076
Coord C2 exon
chr7:4338162-4338300
Length
232 bp
Sequences
Splice sites
3' ss Seq
ACGACAGCAACTCCCCTTAGGCC
3' ss Score
2.8
5' ss Seq
CAGGTAACT
5' ss Score
8.63
Exon sequences
Seq C1 exon
ATGATCTGCCCACGTTGATTACTGGACCGCACCCCAACCAGCCCGACATGCTCGACGTACCTTCTGTTGATGAGGGAACCCCTTACCTCACAAACAATAG
Seq A exon
GCCTGGGTCGAGACGGCAGCCACCAAATGTAGATGAAGCAATTGAAATCCCAGGGTACCAAGTGCCAATCATAGTTGTACCTTCATACCCCCACTCCCGTGAGCCCAGACGCAACGACACCACGGGCCAAGAAGCTCTTTCTCAGACAACCATCTCCTGGAGGCCTTTGCTGGAGAGCACTGAGTACATCATCTCATGCCAACCAGTCAGCCAGGATGAAGATACTTTGCAG
Seq C2 exon
TTCAGGGTTCCTGGCACTTCCTCCAGTGCTACGCTCACTGGCCTCACCAGAGGTGCCACCTACAACATCATAGTAGAAGCCCTGAAGGATCACCGCAGACAAAAGGTGCTGGAGGAGGTGGTCACAGTTGGCAATACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000003578-'58-61,'58-59,59-61
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.876 A=0.664 C2=0.138
Domain overlap (PFAM):
C1:
NO
A:
PF0004116=fn3=PU(54.5=53.8)
C2:
PF0004116=fn3=PD(42.9=70.2)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGATCTGCCCACGTTGATTACTG
R:
GTATTGCCAACTGTGACCACCT
Band lengths:
236-468
Functional annotations
There are 1 annotated functions for this event
PMID: 11246004
The alternatively spliced IIICS segment possesses the major alpha4beta1 integrin-binding sites. The exon is associated to HsaALTA0003426 and other complex AS events.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]