GgaINT0080664 @ galGal4
Intron Retention
Gene
ENSGALG00000016263 | EYS
Description
eyes shut homolog (Drosophila) [Source:HGNC Symbol;Acc:HGNC:21555]
Coordinates
chr3:84963704-84964204:+
Coord C1 exon
chr3:84963704-84963870
Coord A exon
chr3:84963871-84964059
Coord C2 exon
chr3:84964060-84964204
Length
189 bp
Sequences
Splice sites
5' ss Seq
AAGGTAAGA
5' ss Score
10.57
3' ss Seq
TATATATGTTTCATTGAAAGGTG
3' ss Score
7.05
Exon sequences
Seq C1 exon
GTCTGAATGGAGATGATTTTTTGGTATTAGGCCTATGTGATGGCAGAGTGGTATATAGCTACAATCTAGGTTCTGGCACAGCAACAATCATTAGCAAACCACTTGACTTGACACTCAATATTCATGTCATCCATCTTGGCAGATATCTCCAGAAAGGCTGGCTGAAG
Seq A exon
GTAAGAAATTGTTTACTTTAGAAGGGTACACATTTAGATGATAAGTGTGGGGTTTTTTTTCCTCCATATTAAGATGTAAAAATGACAGCTTTTATGTTTCCCTGTTAAGTTTGGAGGGCTCTCTTCATTAGTATTTCCCAAATAAAGTAAAATCCTTTGTTGTAACAAATATATATGTTTCATTGAAAG
Seq C2 exon
GTGGATGATCAGAAGAATAAAACTACCACTTCTCCTGGAAGATTGGTTGGACTCAATGTCTTCAGCCAGTTTTATTTAGGGGGCTATCATGAGTATACTCCAGAACTCCTACCCAAGGGATCCAGATTTAAGAATGGCTTTCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016263:ENSGALT00000026224:8
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0221019=Laminin_G_2=FE(42.0=100)
A:
NA
C2:
PF0221019=Laminin_G_2=FE(36.6=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGGCCTATGTGATGGCAGAGT
R:
CTTGGGTAGGAGTTCTGGAGT
Band lengths:
256-445
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]