GgaINT0096247 @ galGal4
Intron Retention
Gene
ENSGALG00000016037 | SPAG1
Description
sperm associated antigen 1 [Source:HGNC Symbol;Acc:HGNC:11212]
Coordinates
chr2:128193338-128194024:+
Coord C1 exon
chr2:128193338-128193543
Coord A exon
chr2:128193544-128193924
Coord C2 exon
chr2:128193925-128194024
Length
381 bp
Sequences
Splice sites
5' ss Seq
TAGGTGAGT
5' ss Score
8.83
3' ss Seq
GTAATTGTAACTTCTTTTAGGAG
3' ss Score
7.06
Exon sequences
Seq C1 exon
CAACCAAACAAGTGAAAGCCCTGAAGCTGGCCAGTCCTCCAGATCGCATGGAGCAGGGTCCCTGTCAGCTGGTGCTGCCCATTCGACTTCTCTTCCTCCAGCTGCTGCAAAACTGAAAAGCGAAGGAAACGAGTTATTTAAGAGTGGGCAGTTTGGAGAAGCTGTGCCCAAATACTCGGAAGCAATTGAGTATGTCATCAGTGTAG
Seq A exon
GTGAGTGTGATACGTTATACTTAAATTTAGAATTGTTGTTTATGTGATTTAAAGGAACTGCAGATGAACTAACCTAGCCTGTCCCTGGATTTTTGGCATGAGTTACTGTGCAGTGAATACTTAGTCATTGCTAGTAGAGGGTAATTCATGTAATACAAAATGAGGAAGAGGAGTAAAATATTCTTTTAACTTTCTGGGGACAAAGATGTTTGCATTTCTTTGTGTTTGAATAACTAAGTATTCTAGGTGACATAATAAAGGAAAGCTCCTTCCAGTTTTGTATCAAGAGCAGACTGCATCGCTGCAGTACAACTTACTGTATACAATTGCTTGAGTGTCATTTTAGTAATGGTCTTTGATTGTAATTGTAACTTCTTTTAG
Seq C2 exon
GAGAACGAAGTCCAGATGATTTAAGTATCTTGTACTCAAACAGAGCAGCGTGTTACTTAAAAGAAGGCAACTGCAGTGACTGTGTTCAAGATTGTAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000016037:ENSGALT00000025838:13
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.586 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF134141=TPR_11=PU(47.4=51.4)
A:
NA
C2:
PF134141=TPR_11=FE(43.4=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAACCAAACAAGTGAAAGCCCTG
R:
TACAATCTTGAACACAGTCACTGCA
Band lengths:
302-683
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]