GgaINT0119197 @ galGal4
Intron Retention
Gene
ENSGALG00000015358 | myosin
Description
myosin heavy chain, cardiac muscle isoform [Source:RefSeq peptide;Acc:NP_990097]
Coordinates
chr1:86895018-86896820:-
Coord C1 exon
chr1:86896431-86896820
Coord A exon
chr1:86895145-86896430
Coord C2 exon
chr1:86895018-86895144
Length
1286 bp
Sequences
Splice sites
5' ss Seq
AAAGTAAGG
5' ss Score
6.6
3' ss Seq
CTCTTCTGATTCCTATTTAGGCA
3' ss Score
9.01
Exon sequences
Seq C1 exon
GCTCGTATAGAAGAGCTGGAAGAGGAGCTGGAAGCAGAAAGAGCTGCTCGAGCCAAGGTGGAAAAGCAGAGATCAGATTTGGCCCGAGAGCTGGAGGAATTAAGTGAGCGGCTTGAAGAGGCTGGGGGTGCCACTGCTGCCCAGCTGGAGATGAACAAGAAACGTGAAGCTGAGTTCCTGAAGCTGCGGCGTGACCTCGAGGAGGCCACGCTGCACTATGAAGCCACAGCTGCTGCTCTGAGGAAGAAGCATGCGGACAGCGTGGCTGAGATGGGGGAGCAGCTGGACAACCTGCAGCGCGTCAAGCAGAAACTGGAAAAGGAGAAAAGCGAGCTGAAAATGGAAGTGGATGATCTGACATCCAACATGGAGCAAACGGTTAAGGGAAAA
Seq A exon
GTAAGGGCCTTGAGACTTCCCAAACTCCCCAATGATCATGTGTAGCCAAGGCTTGTCCGAAGGCTTCCCAGTAGAGCTGGTAGGGCTGGCATGTTTCAGAAAGTTGTATCTTGCAATCTGGTGGTGCTTGTGTGTGCAGTATCACCCTTTCCCCAGCTGGTGGTATAGCCCTTCTCACTTGGAGACAGAATAGCCATTGAAATGGCCTATCTCAGATGTAGTACATCCTCAGTCCCAGACATACTGGTTATTCACATGTCTCAGAGCACAGAACCATGCCGCTTAAATAGTGCTCACCTAGGCTGATGATGACTGCAGTGATGCCATTCAAGGCTGTACAGCTCAGATGGTGATCTTGGCTCACAGTTTTGACTCACATTAGCAAGGAATGTAAAACATGACCACATGACTCGCATGACCATTCTGTTCATTTTCACTTCCTATGGCTGAATTATGCATTTTGCTTTCTCAGTAGACAGTCTTGGCAGCTTTCAGAGCACTCTAAAGAGTCTGCTCTGTGAAACTGAAGACAGGATTTCACTATTCTGAGTACAAGAAGGCTGACATCAAACTCAATTTTTGGTAAATAGCAATTACAGCAGAATCAGTAAAGCAGTTTTCACTGGAGATGATTTTCCCATCTAACAACTGTGATGCAGCTTTTGTCTGAACTTCATAGGCATATGATTGGTTCCAAAGGTCTTGCAGAGATTTTGTCTCTGGTGCTTCACTTCAGAACACAGCAAATGAGAAAGGTGTTCTGATGGGGAGACCCCTGCCTGGGACCACAGACACAGGGTTTCTATTCCCCATTTTACTGCTGTCCTCCAAAGTGACATGGCAGCTTTTCTTCCCACAGTTCCAGTGTCCTTGTCTTCAAAACAGTGACAATATGTGCACACACACACAGCCCTGATGAGCGGGATTATTTCTTCTTGATTACTTTTAAAACTGAGCCAAATCTCAAATTCTTTATTCATGTTCCAGGCACTCAGTGTTCCTGTAAGGCGCATTTCATTGTTGTCTTATTTGGTGGAAAACAGAAAAGTAGTATTTGCTAGGTCAAATTGCTCTTTAAACCAGGATGCCAATCGAACATGCTATAACAGTTCTTGCTGTAAAGCTTATGAAGATCAGGCCTTACCCTCTCATCAACATTCACTACATGCTGGTGGTGGAAGAGATATTTGTGTCTTTGTTATCTGCTGTTTATTTGAAAGGGGCACAGCTTCCAAATGGCTACTTGGAGGCTGCAGCTTTGTATGTCTCTTCTGATTCCTATTTAG
Seq C2 exon
GCAAATGCAGAAAAACTTTGTCGCACTTATGAAGATCATCTTAATGAGACAAAAACTAAACTGGATGAAATGACTCGCCTCATGAATGACCTCACTACTCAAAAGACAAAACTCCAGAGTGAGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000015358:ENSGALT00000036887:28
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.708 A=NA C2=1.000
Domain overlap (PFAM):
C1:
PF0157614=Myosin_tail_1=FE(24.6=100),PF0003816=Filament=PU(21.9=25.4)
A:
NA
C2:
PF0157614=Myosin_tail_1=FE(8.0=100),PF0003816=Filament=FE(27.8=100),PF0003816=Filament=PU(1.9=14.0)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GCCCAGCTGGAGATGAACAAG
R:
GGTCATTCATGAGGCGAGTCA
Band lengths:
343-1629
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]