GgaINT1017725 @ galGal4
Intron Retention
Gene
ENSGALG00000014699 | MEGF10
Description
multiple EGF-like-domains 10 [Source:HGNC Symbol;Acc:HGNC:29634]
Coordinates
chrZ:56262994-56264341:+
Coord C1 exon
chrZ:56262994-56263122
Coord A exon
chrZ:56263123-56264104
Coord C2 exon
chrZ:56264105-56264341
Length
982 bp
Sequences
Splice sites
5' ss Seq
AAGGTAGAG
5' ss Score
6.38
3' ss Seq
CATTTCTTTTTGTATTTCAGCTG
3' ss Score
9.95
Exon sequences
Seq C1 exon
AATGCCCTCAAGGTACATATGGGTATGGATGTCGGCAGATATGTGACTGTCTGAACAACTCAACCTGTGACCACATCACAGGAACGTGTTACTGCAGCCCAGGCTGGAAGGGGGCCAGGTGTGATCAAG
Seq A exon
GTAGAGACTAGATGCAGTCAAATTACTACAGATGTTTGCTGTAAAACTTAGTCAACCTATTAAATGCCACATATGTGTCAGCCACTAAATTATTTTTTATGTTTTTAATATGTTTCAGTAGTCTGTTATTGAATTATATATATATTCAATAGATTTGTAAGTATTAAATGCCTCAGTGATAGTTTATTGGATCAAATCACTCAGGTGCAGATCGCATGAAATGTTTTACAGTGCTGCGCTGTCCAAAGCTGCTGATAGAGCATATATTTACTCTGTACTTTTGCCTACAAGATGTTCTCACTTTTTCAGCCCTTTCTATGGATCACAGAGTCATAGCAATGGCTTGGGTTGGATGGGATCTCAAGGATCATCAAGTTCCAACTCCTCTTCCACAGGCAGGATTGCCAGCAGCTAGATCATCTACTAGACCAGGCTGCCCAGGGCCCCATCCAACCTGGCCTTAAACACCTCCAGAGACGGGGCATCCACAGCCTGTCTGGACAGCCTGTTCTAGCACCTTACCACTCTCTCTGTAAAAAACTTCCCCGACATCTAATCTAAACCTCCCCTTCTTTAGTTTAAAACCATTCCCCCTTGTCCTGTCACTATCTACCCATGTAAAAAGCTGATTTCCCTCATGTTTATAAACTCCCTTTAAATACTGGAAGGCCACGATGAGATTTCCCCACAGACTTTTCTTCCCTGGGCTGAACAAACCCAGTTCATTCACCCTGTCTTCATAGGAGAGGTACTCCAGCCCTTGGATCATCTTTGTGGCCCTCCTCTGGACCCTCTCCAAAAAGATTAATGCTTTTCCTGAAGGCTAAGGTTGTTTTTTGCATTGGTTTTGGACGTGGTTCTATTTTGGCACTAAAAAATACAGTTAAAATGATCAGATACAGAAGTGAAATACCATCTTCTGCTCCTGACACATAAGCCTCTGAATTTATGCAGTGTATTTTCATTTCTTTTTGTATTTCAG
Seq C2 exon
CTGGCGTAATTATAGTGGGAAACCTGAACAGCTTAAGCCGTACCAGTACTGCCATCCCTGCTGACTCTTACCAGATAGGGGCTATAGCAGGCATCATCATTCTTGTCTTGATTGTGCTCTTCCTGCTAGTGCTGTTCATTATTTACAGACATAAGCAAAAAGGGAAAGAAACAAACATGCCTGCAGTGACCTATACTCCTGCGATGAGGGTCATCAATGCTGATTACACCATTTCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSGALG00000014699:ENSGALT00000023694:19
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.013
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(34.8=36.4),PF0005319=Laminin_EGF=PU(78.4=65.9)
A:
NA
C2:
PF0005319=Laminin_EGF=PD(18.9=8.8),PF061436=Baculo_11_kDa=WD(100=51.2)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGCCCTCAAGGTACATATGGGT
R:
GATGACCCTCATCGCAGGAGT
Band lengths:
342-1324
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]