HsaEX0052689 @ hg19
Exon Skipping
Gene
ENSG00000102317 | RBM3
Description
RNA binding motif (RNP1, RRM) protein 3 [Source:HGNC Symbol;Acc:9900]
Coordinates
chrX:48433949-48434807:+
Coord C1 exon
chrX:48433949-48434055
Coord A exon
chrX:48434203-48434471
Coord C2 exon
chrX:48434702-48434807
Length
269 bp
Sequences
Splice sites
3' ss Seq
ATGACTGCCTTTTGTTCTAGGGG
3' ss Score
10.49
5' ss Seq
GATGTAAGT
5' ss Score
9.11
Exon sequences
Seq C1 exon
TGGTCGTTGTCAAGGACCGGGAGACTCAGCGGTCCAGGGGTTTTGGTTTCATCACCTTCACCAACCCAGAGCATGCTTCAGTTGCCATGAGAGCCATGAACGGAGAG
Seq A exon
GGGGTGGAGGGCAGCGGCAGACAGAGCCTGGCCGCCTGGGAGGCGACGACTGGTCCTGCATGAGGCCGAGAAGCCACCTGTGGATGGTTGACCTGCCTTGGGCTTAGGTAGTAGAGTCTGCAGACCTGTGGGCCTGGCCCGGAGAGGACCTCGTGAGTCTTCTGACATGGACTCTGAAGCCTCAAACCAAGAGAGGTGTCTGTCTAGCCCTGATAGTCGGGCAGCTTAGAGGAGTGGGGAGGAGAACTCAGCCTGAGTTCAGTCAAGAT
Seq C2 exon
TCTCTGGATGGTCGTCAGATCCGTGTGGATCATGCAGGCAAGTCTGCTCGGGGAACCAGAGGAGGTGGCTTTGGGGCCCATGGGCGTGGTCGCAGCTACTCTAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSG00000102317_MULTIEX1-2/2=1-C2
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.329 A=0.950 C2=0.565
Domain overlap (PFAM):
C1:
PF0007617=RRM_1=FE(50.7=100),PF093415=Pcc1=PU(51.4=52.8)
A:
PF0007617=RRM_1=PD(7.2=23.8),PF093415=Pcc1=PD(43.2=76.2)
C2:
PF0007617=RRM_1=PD(9.9=19.4)
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGTCAAGGACCGGGAGACTC
R:
TCTAGAGTAGCTGCGACCACG
Band lengths:
206-475
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
This event
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.817, FDR=0.004), PC9 No Change at 14 days (FC=0.874, FDR=0.114), Late Xenograft Enriched (FC=1.769, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- The Cancer Genome Atlas (TCGA)
- Genotype-Tissue Expression Project (GTEx)
- Autistic and control brains
- Pre-implantation embryo development
Other AS DBs:
FasterDB (Includes CLIP-seq data)
AS-ALPS (AS-induced ALteration of Protein Structure, links to PINs)
APPRIS (Selection of principal isoform)
DEU primates (Only for human)