BtaEX0029920 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000025848 | RBM3
Description
RNA binding motif (RNP1, RRM) protein 3 [Source:HGNC Symbol;Acc:HGNC:9900]
Coordinates
chrX:91769034-91769886:+
Coord C1 exon
chrX:91769034-91769140
Coord A exon
chrX:91769284-91769552
Coord C2 exon
chrX:91769781-91769886
Length
269 bp
Sequences
Splice sites
3' ss Seq
GTGACTGCCTTCTGTTCTAGGGG
3' ss Score
11.07
5' ss Seq
GATGTAAGT
5' ss Score
9.11
Exon sequences
Seq C1 exon
TGGTGGTCGTCAAGGACCGGGAGACTCAGCGATCCCGGGGTTTTGGCTTCATCACCTTCACCAATCCGGAGCATGCCTCAAATGCCATGAGAGCCATGAATGGAGAG
Seq A exon
GGGGTGGAGGGCAGCGGCAGACAGAGCCGGGCTGCCTGGGAGGCGACGACTGGTCCTGCATGAGGCCGAGAAGCCACCTGTGGATGGTTGACCTGCTCTGGGCTTAGGTAGTAGAGTCTGCAGACCTGCGGGCCTGGCCCGGAGAGGACCTCGTGAGTCTTCTGGGATGGACTCTGAAGCCTCAGACCAAGAGAGGTGTCTGTCTAGCCCTAATAGTCGGGCAGCTTAGAGGAGTGGGGAGGAGAATTCAACCTGAGTTCGGTCAAGAT
Seq C2 exon
TCTCTGGATGGTCGTCAGATCCGTGTAGACCACGCTGGCAAGTCAGCCCGGGGATCACGAGGGGGTGCCTTTGGGGGCTATGAACGTGGTCGTGGCTACCCTAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000025848_CASSETTE1
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.542 A=1.000 C2=0.972
Domain overlap (PFAM):
C1:
PF0007617=RRM_1=FE(49.3=100)
A:
PF0007617=RRM_1=PD(7.2=23.8)
C2:
PF0007617=RRM_1=PD(9.9=19.4)
Main Inclusion Isoform:
ENSBTAT00000024034fB5810
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTCAAGGACCGGGAGACTCAG
R:
CTCTAGGGTAGCCACGACCAC
Band lengths:
205-474
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.817, FDR=0.004), PC9 No Change at 14 days (FC=0.874, FDR=0.114), Late Xenograft Enriched (FC=1.769, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development