RnoEX0074637 @ rn6
Exon Skipping
Gene
ENSRNOG00000005387 | Rbm3
Description
RNA binding motif protein 3 [Source:RGD Symbol;Acc:620145]
Coordinates
chrX:15099953-15100804:+
Coord C1 exon
chrX:15099953-15100059
Coord A exon
chrX:15100206-15100470
Coord C2 exon
chrX:15100699-15100804
Length
265 bp
Sequences
Splice sites
3' ss Seq
ATGACTGCCTTCTGTTCTAGGGG
3' ss Score
11.31
5' ss Seq
GATGTAAGT
5' ss Score
9.11
Exon sequences
Seq C1 exon
TGGTTGTTGTCAAGGACCGGGAGACTCAAAGATCCCGGGGTTTTGGCTTCATCACCTTCACAAACCCAGAGCATGCCTCCGATGCCATGAGAGCCATGAATGGAGAG
Seq A exon
GGGGTGGAGGGCAGCGGCAGACAGAGCCGGGCTGCCTGGGAGGCGACGACTGGTCCTGCATGAGGCCGAGAAGCCACCTGTGGATGGTTGACCTGCTCTGGGCTTAGGTAGTAGAGTCTGCAGACCTGTGGGCCTGGCCCGGAGAGGACCTCGTGAGTCTGCTGGGATGGACTCTGAAGCCTCAAACCAAGAGAGGCGTCTAGCCCTAATAGTCGGGCAGCTTAGAGGAGTGGGGAGGAGAACTCAGCCTGAGTTCAGTCAAGAT
Seq C2 exon
TCCCTGGATGGGCGCCAAATCCGTGTGGACCATGCAGGCAAGTCTGCCAGGGGAACCAGAGGGGGTGCCTTTGGGGCCCATGGGCGTGGTCGCAGCTACTCTAGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000005387_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.439 A=1.000 C2=0.586
Domain overlap (PFAM):
C1:
PF0007617=RRM_1=PD(57.6=33.0)
A:
PF0007617=RRM_1=PD(7.2=23.8)
C2:
PF0007617=RRM_1=PD(9.9=19.4)
Main Inclusion Isoform:
ENSRNOT00000007367fB12255
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGTTGTTGTCAAGGACCGG
R:
TCTAGAGTAGCTGCGACCACG
Band lengths:
212-477
Functional annotations
There are 1 annotated functions for this event
PMID: 31911676
[CRISPR screen]. Conserved poison exon with pro-oncogenic impact when depleted: HeLa No Change at 14 days (FC=0.817, FDR=0.004), PC9 No Change at 14 days (FC=0.874, FDR=0.114), Late Xenograft Enriched (FC=1.769, FDR=0.000).
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]