MmuALTD0011589-2/2 @ mm10
Alternative 5'ss
Gene
ENSMUSG00000004952 | Rasa4
Description
RAS p21 protein activator 4 [Source:MGI Symbol;Acc:MGI:1858600]
Coordinates
chr5:136101251-136101689:+
Coord C1 exon
chr5:136101251-136101394
Coord A exon
chr5:136101395-136101420
Coord C2 exon
chr5:136101625-136101689
Length
26 bp
Sequences
Splice sites
5' ss Seq
CAGGCAGGG
5' ss Score
1.71
3' ss Seq
ACTAGAATTTCTGCTTGCAGGTG
3' ss Score
7.42
Exon sequences
Seq C1 exon
GACGGCTGATTCCTGTCATTGAGGAGACAACTAGTGCCGAGTGTCGCCAGGAAGTGGCCACCACCCTGCTCAAACTCTTCCTGGGGCAGGGGCTGGCCAAGGACTTTCTGGATCTGCTTTTTCAGCTGGAGCTGGGCCGCACCA
Seq A exon
GTGAGGCCTGAGAGGACTTGTCCCAG
Seq C2 exon
GTGAAGCCAACACCCTCTTCCGGAGCAATTCTCTGGCCTCAAAGTCCATGGAGTCTTTCCTGAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000004952-22-23,23-23-2/2
Average complexity
Alt5
Mappability confidence:
NA
Protein Impact
ORF disruption when splice site is used (sequence inclusion)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0061614=RasGAP=PU(6.8=25.5)
A:
NA
C2:
PF0061614=RasGAP=FE(11.1=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Associated events
Other assemblies
Conservation
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCACCCTGCTCAAACTCTTCC
R:
GAATTGCTCCGGAAGAGGGTG
Band lengths:
115-141
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types